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GRP94 antibody [N1N3]

Anti-GRP94 antibody [N1N3] used in Western Blot (WB). GTX103203
Anti-GRP94 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103203
Anti-GRP94 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103203
Anti-GRP94 antibody [N1N3] used in Western Blot (WB). GTX103203
Anti-GRP94 antibody [N1N3] used in Immunoprecipitation (IP). GTX103203
Anti-GRP94 antibody [N1N3] used in Western Blot (WB). GTX103203

Cat No. GTX103203

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3 , *endothelial cells

Predict Reactivity

Rat, Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GRP94. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

heat shock protein 90 beta family member 1 , ECGP , GP96 , GRP94 , HEL-S-125m , HEL35 , TRA1

Cellular Localization

Endoplasmic reticulum lumen , Melanosome

Background

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-GRP94 antibody [N1N3] used in Western Blot (WB). GTX103203

GTX103203 WB Image

Wild-type (WT) and GRP94 knockout (KO) 293T cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP94 antibody [N1N3] (GTX103203) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRP94 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103203

GTX103203 ICC/IF Image

GRP94 antibody [N1N3] detects GRP94 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GRP94 protein stained by GRP94 antibody [N1N3] (GTX103203) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-GRP94 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103203

GTX103203 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCM-1 xenograft , using HSP90B(GTX103203) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GRP94 antibody [N1N3] used in Western Blot (WB). GTX103203

GTX103203 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela S3
7.5% SDS PAGE
GTX103203 diluted at 1:1000

Anti-GRP94 antibody [N1N3] used in Immunoprecipitation (IP). GTX103203

GTX103203 IP Image

Immunoprecipitation of GRP94 protein from HeLa whole cell extracts using 5 μg of GRP94 antibody [N1N3] (GTX103203).
Western blot analysis was performed using GRP94 antibody [N1N3] (GTX103203).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-GRP94 antibody [N1N3] used in Western Blot (WB). GTX103203

GTX103203 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX103203 diluted at 1:5000

REFERENCE
REVIEW

GRP94 antibody [N1N3] Cat No. GTX103203

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 25-Dec-2014
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : endothelial cells
Blocking : 10% goat serum in PBS, 25°C, 1Hr
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : 0.2% Triton X100, 25°C, 10minHr
Primary Antibody : 1:200, 4°C, 17Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169