Wild-type (WT) and GRP94 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP94 antibody [N1N3] (GTX103203) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX103203 diluted at 1:5000

Immunohistochemical analysis of paraffin-embedded SCM-1 xenograft , using HSP90B(GTX103203) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela S3
7.5% SDS PAGE
GTX103203 diluted at 1:1000

GRP94 antibody [N1N3] detects GRP94 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GRP94 protein stained by GRP94 antibody [N1N3] (GTX103203) diluted at 1:500.
Blue: Hoechst 33342 staining.

Immunoprecipitation of GRP94 protein from HeLa whole cell extracts using 5 μg of GRP94 antibody [N1N3] (GTX103203).
Western blot analysis was performed using GRP94 antibody [N1N3] (GTX103203).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

The data was published in the journal Elife in 2016.PMID: 27435961

The data was published in the journal Front Neurol in 2019.PMID: 31258504

The data was published in the 2022 in Orphanet J Rare Dis. PMID: 35101074