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GRP94 antibody [SPM249]

Anti-GRP94 antibody [SPM249] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34772
Anti-GRP94 antibody [SPM249] used in IHC (Paraffin sections) (IHC-P). GTX34772
Anti-GRP94 antibody [SPM249] used in Flow cytometry (FACS). GTX34772

Cat. No. GTX34772

Host

 Rat

Clonality

 Monoclonal

Clone Name

 SPM249

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Hamster, Chicken, Guinea pig, Pig, Monkey, Xenopus laevis, Horse
Package
100 μg ($529)
See all GRP94 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Product Note

This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Chicken

Immunogen

Purified glucose regulated protein 94 (grp94) from chicken oviducts

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Endoplasmic reticulum lumen

Database

Research Area

DATA IMAGES

Anti-GRP94 antibody [SPM249] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34772

GTX34772 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX34772 GRP94 antibody [SPM249].
Green : Primary antibody
Red : nucleus

Anti-GRP94 antibody [SPM249] used in IHC (Paraffin sections) (IHC-P). GTX34772

GTX34772 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX34772 GRP94 antibody [SPM249].

Anti-GRP94 antibody [SPM249] used in Flow cytometry (FACS). GTX34772

GTX34772 FACS Image

FACS analysis of PFA-fixed HepG2 cells using GTX34772 GRP94 antibody [SPM249].
Blue : Primary antibody
Red : Isotype control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529