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GRP94 antibody

Anti-GRP94 antibody used in Western Blot (WB). GTX103232
Anti-GRP94 antibody used in Western Blot (WB). GTX103232
Anti-GRP94 antibody used in IHC (Paraffin sections) (IHC-P). GTX103232
Anti-GRP94 antibody used in Western Blot (WB). GTX103232
Anti-GRP94 antibody used in Western Blot (WB). GTX103232
Anti-GRP94 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103232
Anti-GRP94 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103232
Anti-GRP94 antibody used in Immunoprecipitation (IP). GTX103232
Anti-GRP94 antibody used in IHC (Paraffin sections) (IHC-P). GTX103232

Cat No. GTX103232

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH3T3 , PC-12 , Rat-2

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GRP94. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

heat shock protein 90 beta family member 1,ECGP,GP96,GRP94,HEL-S-125m,HEL35,TRA1

Cellular Localization

Endoplasmic reticulum lumen , Melanosome

Background

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-GRP94 antibody used in Western Blot (WB). GTX103232

GTX103232 WB Image

Wild-type (WT) and GRP94 knockout (KO) 293T cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP94 antibody (GTX103232) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRP94 antibody used in Western Blot (WB). GTX103232

GTX103232 WB Image

GRP94 antibody detects GRP94 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5 % SDS-PAGE, and blotted with GRP94 antibody (GTX103232) diluted by 1:1000

Anti-GRP94 antibody used in IHC (Paraffin sections) (IHC-P). GTX103232

GTX103232 IHC-P Image

GRP94 antibody detects GRP94 protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
GRP94 antibody (GTX103232) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GRP94 antibody used in Western Blot (WB). GTX103232

GTX103232 WB Image

GRP94 antibody detects GRP94 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with GRP94 antibody (GTX103232) diluted by 1:1000

Anti-GRP94 antibody used in Western Blot (WB). GTX103232

GTX103232 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP94 antibody (GTX103232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRP94 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103232

GTX103232 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GRP94(GTX103232) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:2500.

Anti-GRP94 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103232

GTX103232 ICC/IF Image

GRP94 antibody detects GRP94 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GRP94 protein stained by GRP94 antibody (GTX103232) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-GRP94 antibody used in Immunoprecipitation (IP). GTX103232

GTX103232 IP Image

Immunoprecipitation of GRP94 protein from HeLa whole cell extracts using 5 μg of GRP94 antibody (GTX103232).
Western blot analysis was performed using GRP94 antibody (GTX103232).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-GRP94 antibody used in IHC (Paraffin sections) (IHC-P). GTX103232

GTX103232 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HEP3B xenograft, using GRP94(GTX103232) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169