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GRP94 antibody

KO/KD Orthogonal
Anti-GRP94 antibody used in Western Blot (WB). GTX103232
Anti-GRP94 antibody used in Western Blot (WB). GTX103232
Anti-GRP94 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103232
Anti-GRP94 antibody used in Immunoprecipitation (IP). GTX103232
Anti-GRP94 antibody used in Western Blot (WB). GTX103232
Anti-GRP94 antibody used in Western Blot (WB). GTX103232
Anti-GRP94 antibody used in IHC (Paraffin sections) (IHC-P). GTX103232
Anti-GRP94 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103232
Anti-GRP94 antibody used in IHC (Paraffin sections) (IHC-P). GTX103232

Cat. No. GTX103232

Reference 16
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GRP94 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

PC-12 , Rat-2 , 293T , A431 , HeLa , HepG2 , NIH3T3

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GRP94. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950516

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein 90 beta family member 1 , ECGP , GP96 , GRP94 , HEL-S-125m , HEL35 , TRA1

Cellular Localization

Endoplasmic reticulum lumen , Melanosome

Background

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-GRP94 antibody used in Western Blot (WB). GTX103232

GTX103232 WB Image

KO/KD

Wild-type (WT) and GRP94 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP94 antibody (GTX103232) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRP94 antibody used in Western Blot (WB). GTX103232

GTX103232 WB Image

GRP94 antibody detects GRP94 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5 % SDS-PAGE, and blotted with GRP94 antibody (GTX103232) diluted by 1:1000

Anti-GRP94 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103232

GTX103232 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GRP94(GTX103232) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:2500.

Anti-GRP94 antibody used in Immunoprecipitation (IP). GTX103232

GTX103232 IP Image

Immunoprecipitation of GRP94 protein from HeLa whole cell extracts using 5 μg of GRP94 antibody (GTX103232).
Western blot analysis was performed using GRP94 antibody (GTX103232).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-GRP94 antibody used in Western Blot (WB). GTX103232

GTX103232 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP94 antibody (GTX103232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRP94 antibody used in Western Blot (WB). GTX103232

GTX103232 WB Image

GRP94 antibody detects GRP94 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with GRP94 antibody (GTX103232) diluted by 1:1000

Anti-GRP94 antibody used in IHC (Paraffin sections) (IHC-P). GTX103232

GTX103232 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HEP3B xenograft, using GRP94(GTX103232) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GRP94 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103232

GTX103232 ICC/IF Image

GRP94 antibody detects GRP94 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GRP94 protein stained by GRP94 antibody (GTX103232) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-GRP94 antibody used in IHC (Paraffin sections) (IHC-P). GTX103232

GTX103232 IHC-P Image

GRP94 antibody detects GRP94 protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
GRP94 antibody (GTX103232) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Chua HH et al. Cell Mol Gastroenterol Hepatol 2023; 15 (1) : 121-152 PIM1-Induced Cytoplasmic Expression of RBMY Mediates Hepatocellular Carcinoma Metastasis.
Chen HH et al. iScience 2021; 24 (9) : 103086 DDX3 modulates the tumor microenvironment via its role in endoplasmic reticulum-associated translation.
Application : WB
Reactivity : Human
Brzozowa-Zasada M et al. Prz Gastroenterol 2019; 14 (2) : 140-147 The clinical and prognostic evaluation of GRP94 immunoexpression in Caucasian patients with colorectal adenocarcinoma.
Application : IHC-P
Reactivity : Human
Domenis R et al. Sci Rep 2019; 9 (1) : 8457 Toll-like Receptor-4 Activation Boosts the Immunosuppressive Properties of Tumor Cells-derived Exosomes.
Application : WB
Reactivity : Human
Xiao X et al. Toxicol Appl Pharmacol 2019; 373 : 10月25日 HHQ-4, a quinoline derivate, preferentially inhibits proliferation of glucose-deprived breast cancer cells as a GRP78 down-regulator.
Reactivity : Human
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Submit a Reference

REVIEW

GRP94 antibody Cat. No. GTX103232

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-GRP94 antibody used in Western Blot (WB). GTX103232
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 0-30 μM cisplatin treatment for 24hr.
Anti-GRP94 antibody used in Western Blot (WB). GTX103232
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 25811758
Submit a Review
Application Reference
Chua HH et al. Cell Mol Gastroenterol Hepatol 2023; 15 (1):121-152 PIM1-Induced Cytoplasmic Expression of RBMY Mediates Hepatocellular Carcinoma Metastasis.
Chen HH et al. iScience 2021; 24 (9):103086 DDX3 modulates the tumor microenvironment via its role in endoplasmic reticulum-associated translation.
Application : WB
Reactivity : Human
Brzozowa-Zasada M et al. Prz Gastroenterol 2019; 14 (2):140-147 The clinical and prognostic evaluation of GRP94 immunoexpression in Caucasian patients with colorectal adenocarcinoma.
Application : IHC-P
Reactivity : Human
Domenis R et al. Sci Rep 2019; 9 (1):8457 Toll-like Receptor-4 Activation Boosts the Immunosuppressive Properties of Tumor Cells-derived Exosomes.
Application : WB
Reactivity : Human
Xiao X et al. Toxicol Appl Pharmacol 2019; 373:10月25日 HHQ-4, a quinoline derivate, preferentially inhibits proliferation of glucose-deprived breast cancer cells as a GRP78 down-regulator.
Reactivity : Human
Lee CW et al. J Cell Physiol 2018; (Epub) Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species.
Application : WB
Reactivity : Human
Domenis R et al. Sci Rep 2018; 8 (1):13325 Pro inflammatory stimuli enhance the immunosuppressive functions of adipose mesenchymal stem cells-derived exosomes.
Application : WB
Reactivity : Human
Lars Plate et al. bioRxiv 2018; (Epub) Quantitative Interactome Proteomics Reveals a Molecular Basis for ATF6-Dependent Regulation of a Destabilized Amyloidogenic Protein.
Application : WB
Reactivity : Human
Zadoo S et al. Invest Ophthalmol Vis Sci 2016; 57 (4):1939-50 A Novel Luciferase Assay For Sensitively Monitoring Myocilin Variants in Cell Culture.
Application : WB
Reactivity : Human
Boso G et al. PLoS One 2015; 10 (3):e0121455 Characterization of resistance to rhabdovirus and retrovirus infection in a human myeloid cell line.
Application : WB
Reactivity : Human
Hulleman JD et al. FASEB J 2015; 29 (2):565-75 Genetic ablation of N-linked glycosylation reveals two key folding pathways for R345W fibulin-3, a secreted protein associated with retinal degeneration.
Application : WB
Reactivity : Human
Chen DJ et al. Proteomics. 2015; Proteomic analysis of secreted proteins by human bronchial epithelial cells in response to cadmium toxicity.[Epub ahead of print]
Application : WB
Reactivity : Human
Li PC et al. PLoS One 2014; 9 (2):e85909 Inhibition of reverse-mode sodium-calcium exchanger activity and apoptosis by levosimendan in human cardiomyocyte progenitor cell-derived cardiomyocytes after anoxia and reoxygenation.
Application : WB
Reactivity : Human
Krishnan S et al. Microbes Infect 2014; 16 (7):540-52 Identification of minimum carbohydrate moiety in N-glycosylation sites of brain endothelial cell glycoprotein 96 for interaction with Escherichia coli K1 outer membrane protein A.
Application : ICC/IF
Reactivity : Human
Krishnan S et al. J Infect Dis 2014; 209 (3):409-19 Angiotensin II receptor type 1--a novel target for preventing neonatal meningitis in mice by Escherichia coli K1.
Shi-Chen Ou D et al. Cell Res 2011; 21 (4):642-53 Transcriptional activation of endoplasmic reticulum chaperone GRP78 by HCMV IE1-72 protein.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169