GeneTex
United States (US)

GRPR antibody [C1C2], Internal

GTX100015_WB_19011506_525.jpg
GTX100015_39224_20180323_WB_R_pancreas_19011506_290.jpg
GTX100015_39224_20180323_WB_M_pancreas_19011506_195.jpg
GTX100015_39224_20180323_WB_M_R_19011506_287.jpg

Cat No. GTX100015

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

HeLaS3 , Molt-4 , mouse pancreas , mouse brain , rat brain , rat pancreas

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human GRPR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GRPR antibody, GRP-R antibody, gastrin-releasing peptide receptor antibody, GRP-preferring bombesin receptor antibody

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Gastrin-releasing peptide (GRP) regulates numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation and is a potent mitogen for neoplastic tissues. The effects of GRP are mediated through the gastrin-releasing peptide receptor. This receptor is a glycosylated, 7-transmembrane G-protein coupled receptor that activates the phospholipase C signaling pathway. The receptor is aberrantly expressed in numerous cancers such as those of the lung, colon, and prostate. An individual with autism and multiple exostoses was found to have a balanced translocation between chromosome 8 and a chromosome X breakpoint located within the gastrin-releasing peptide receptor gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100015_WB_19011506_525.jpg

GTX100015 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
B:MOLT4(GTX27912)
10% SDS PAGE
GTX100015 diluted at 1:1000

GTX100015_39224_20180323_WB_R_pancreas_19011506_290.jpg

GTX100015 WB Image

Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GRPR antibody [C1C2], Internal (GTX100015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100015_39224_20180323_WB_M_pancreas_19011506_195.jpg

GTX100015 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GRPR antibody [C1C2], Internal (GTX100015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100015_39224_20180323_WB_M_R_19011506_287.jpg

GTX100015 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GRPR antibody [C1C2], Internal (GTX100015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169