ICC/IF analysis of HCT116 cells using GTX00895 GRWD1 antibody. To remove nucleoplasmic proteins, the cells were pre-extracted with Triton X-100 before stained.
Dilution : 1:500

ICC/IF analysis of HeLa cells using GTX00895 GRWD1 antibody.
Fixation : 4% PFA, overnight
Permeabilization : 0.25% Triton X-100 in PBS, 10 min
Dilution : 1:1000

WB analysis of various samples using GTX00895 GRWD1 antibody.
Lane 1 : untreated HeLa cells
Lane 2 : HeLa cells treated with adriamycin
Lane 3 : NIH-3T3 whole cell lysate
Dilution : 1:1000
Loading : 10 μg