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GRWD1 antibody

Anti-GRWD1 antibody used in Western Blot (WB). GTX00895
Anti-GRWD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00895
Anti-GRWD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00895

Cat No. GTX00895

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP, ChIP assay

Reactivity

Human, Mouse, Rat
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified GST-GRWD1 (human, full-length) expressed in E. coli

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glutamate rich WD repeat containing 1 , CDW4 , GRWD , GRWD1 , RRB1 , WDR28

Cellular Localization

Nucleus,Nucleolus,Chromosome

Background

This gene encodes a glutamate-rich protein that contains five WD-repeat motifs. The encoded protein may play a critical role in ribosome biogenesis and may also play a role in histone methylation through interactions with CUL4-DDB1 ubiquitin E3 ligase. [provided by RefSeq, Feb 2012]

Database

DATA IMAGES

Anti-GRWD1 antibody used in Western Blot (WB). GTX00895

GTX00895 WB Image

WB analysis of various samples using GTX00895 GRWD1 antibody.
Lane 1 : untreated HeLa cells
Lane 2 : HeLa cells treated with adriamycin
Lane 3 : NIH-3T3 whole cell lysate
Dilution : 1:1000
Loading : 10 μg

Anti-GRWD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00895

GTX00895 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00895 GRWD1 antibody.
Fixation : 4% PFA, overnight
Permeabilization : 0.25% Triton X-100 in PBS, 10 min
Dilution : 1:1000

Anti-GRWD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00895

GTX00895 ICC/IF Image

ICC/IF analysis of HCT116 cells using GTX00895 GRWD1 antibody. To remove nucleoplasmic proteins, the cells were pre-extracted with Triton X-100 before stained.
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 369