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GS1 antibody

Anti-GS1 antibody used in Western Blot (WB). GTX115073
Anti-GS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115073
Anti-GS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115073

Cat. No. GTX115073

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621343

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pseudouridine 5'-phosphatase , DXF68S1E , FAM16AX , GS1 , HDHD1 , HDHD1A

Background

This gene encodes a member of the haloacid dehalogenase-like (HAD) hydrolase superfamily. The encoded protein has no known biological function. This gene has a pseudogene on chromosome 1. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GS1 antibody used in Western Blot (WB). GTX115073

GTX115073 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GTX115073 diluted at 1:1000

Anti-GS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115073

GTX115073 ICC/IF Image

GS1 antibody detects HDHD1A protein at Cytoplasm and Nucleolus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HDHD1A protein stained by GS1 antibody (GTX115073) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-GS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115073

GTX115073 IHC-P Image

GS1 antibody detects GS1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
GS1 antibody (GTX115073) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169