GeneTex
United States (US)

GS1 antibody

GTX115073_40247_IHC_18121410_678.jpg
GTX115073_40247_IFA_18121410_951.jpg
GTX115073_WB_18121410_808.jpg

Cat No. GTX115073

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

HepG2
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DXF68S1E antibody, FAM16AX antibody, GS1 antibody, HDHD1A antibody, HDHD1 antibody, "family with sequence similarity 16, member A, X-linked antibody", haloacid dehalogenase-like hydrolase domain containing 1A antibody, pseudouridine-5'-monophosphatase antibody, haloacid dehalogenase-like hydrolase domain-containing protein 1 antibody, 5'-PsiMPase antibody, haloacid dehalogenase-like hydrolase domain-containing protein 1A antibody, haloacid dehalogenase-like hydrolase domain containing 1 antibody

Background

This gene encodes a member of the haloacid dehalogenase-like (HAD) hydrolase superfamily. The encoded protein has no known biological function. This gene has a pseudogene on chromosome 1. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]
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Database

DATA IMAGES
GTX115073_40247_IHC_18121410_678.jpg

GTX115073 IHC-P Image

GS1 antibody detects GS1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
GS1 antibody (GTX115073) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115073_40247_IFA_18121410_951.jpg

GTX115073 ICC/IF Image

GS1 antibody detects HDHD1A protein at Cytoplasm and Nucleolus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HDHD1A protein stained by GS1 antibody (GTX115073) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX115073_WB_18121410_808.jpg

GTX115073 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GTX115073 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169