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GSK3 alpha (phospho Ser21) + GSK3 beta (phospho Ser9) antibody

Anti-GSK3 alpha (phospho Ser21) + GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX132997

Cat No. GTX132997

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-Fr
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

NIH3T3 starvation for 16 hr , NIH3T3( starvation for 16 hr and 5 ng/ml PDGF treatment for 15 min)

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human GSK3 beta (phospho Ser9). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glycogen synthase kinase 3 beta

Background

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase (see GYS1, MIM 138570). Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-GSK3 alpha (phospho Ser21) + GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX132997

GTX132997 WB Image

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (18 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 alpha (phospho Ser21) + GSK3 beta (phospho Ser9) antibody (GTX132997) diluted at 1:1000.

REFERENCE
REVIEW

GSK3 alpha (phospho Ser21) + GSK3 beta (phospho Ser9) antibody Cat No. GTX132997

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 20-Mar-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : H1975 cells
Amount used : 30 μg
Blocking : 5% BSA, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169