GSK3 alpha (phospho Ser21) + GSK3 beta (phospho Ser9) antibody

Name: GSK3 alpha (phospho Ser21) + GSK3 beta (phospho Ser9) antibody
Brand: GeneTex
SKU: GTX132997
Price: 399.00 USD
Rating: 3.5 (2 reviews)

Anti-GSK3 alpha (phospho Ser21) + GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX132997

Cat. No. GTX132997

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-Fr
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all GSK3 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-Fr	Assay dependent

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

NIH3T3( starvation for 16 hr and 5 ng/ml PDGF treatment for 15 min)

Predict Reactivity

Rat, Sheep, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser9 of human GSK3 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886803

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glycogen synthase kinase 3 beta

Background

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase (see GYS1, MIM 138570). Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).[supplied by OMIM]

Database

Gene ID: 2932 GSK3B
UniProt: P49841 GSK3B

Research Area

DATA IMAGES

GTX132997 WB Image

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (18 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 alpha (phospho Ser21) + GSK3 beta (phospho Ser9) antibody (GTX132997) diluted at 1:1000.

REFERENCE

Application Reference

Hung SY et al. Front Cell Neurosci 2022; 16 : 880267 Electroacupuncture improves TBI dysfunction by targeting HDAC overexpression and BDNF-associated Akt/GSK-3β signaling.

Liu Y.J. et al. Int. J. Mol. Sci. 2021; 22 (6) : 2823 The Anti-Melanogenesis Effect of 3,4-Dihydroxybenzalacetone through Downregulation of Melanosome Maturation and Transportation in B16F10 and Human Epidermal Melanocytes.

Application : WB

Zuo Z et al. Cell Biol Int 2020; 44 (11) : 2284-2292 LncRNA TTN-AS1/miR-134-5p/PAK3 axis regulates the radiosensitivity of human large intestine cancer cells through the P21 pathway and AKT/GSK-3β/β-catenin pathway.

Application : WB

Liu J et al. Oncol Lett 2017; 14 (5) : 5711-5718 miR-214 targets the PTEN-mediated PI3K/Akt signaling pathway and regulates cell proliferation and apoptosis in ovarian cancer.

Leibinger M et al. Proc Natl Acad Sci U S A 2017; 114 (27) : E5454-E5463 Boosting CNS axon regeneration by harnessing antagonistic effects of GSK3 activity.

Application : IHC-Fr

Reactivity : Mouse

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