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GSK3 alpha (phospho Ser21) + GSK3 beta (phospho Ser9) antibody

Anti-GSK3 alpha (phospho Ser21) + GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX132997

Cat No. GTX132997

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-Fr

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

NIH3T3 starvation for 16 hr , NIH3T3( starvation for 16 hr and 5 ng/ml PDGF treatment for 15 min)

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human GSK3 alpha (phospho Ser21) + GSK3 beta (phospho Ser9). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886803

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glycogen synthase kinase 3 beta

Background

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase (see GYS1, MIM 138570). Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-GSK3 alpha (phospho Ser21) + GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX132997

GTX132997 WB Image

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (18 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 alpha (phospho Ser21) + GSK3 beta (phospho Ser9) antibody (GTX132997) diluted at 1:1000.

REFERENCE

REVIEW

GSK3 alpha (phospho Ser21) + GSK3 beta (phospho Ser9) antibody Cat No. GTX132997

Rating ( Average 3.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 3.5 based on 2 users reviews)
Date : Anonymous submitted on 05-Dec-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 5μg
Blocking : 5% BSA in TBS-T, RT, 1Hr
Primary Antibody : 1:2000, 25°C, 1Hr
Lane Description : The same sample was loaded into 3 lanes.
Date : Anonymous submitted on 20-Mar-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : H1975 cells
Amount used : 30 μg
Blocking : 5% BSA, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169