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GSK3 alpha (phospho Ser21) antibody

Anti-GSK3 alpha (phospho Ser21) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32191
Anti-GSK3 alpha (phospho Ser21) antibody used in IHC (Paraffin sections) (IHC-P). GTX32191
Anti-GSK3 alpha (phospho Ser21) antibody used in Western Blot (WB). GTX32191

Cat. No. GTX32191

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all GSK3 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of GSK3 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycogen synthase kinase 3 alpha

Background

This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-GSK3 alpha (phospho Ser21) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32191

GTX32191 ICC/IF Image

ICC/IF analysis of formalin-fixed PC12 cells using GTX32191 GSK3 alpha (phospho Ser21) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-GSK3 alpha (phospho Ser21) antibody used in IHC (Paraffin sections) (IHC-P). GTX32191

GTX32191 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32191 GSK3 alpha (phospho Ser21) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-GSK3 alpha (phospho Ser21) antibody used in Western Blot (WB). GTX32191

GTX32191 WB Image

WB analysis of UV-treated HEK293T (A), mouse liver (B), rat liver (C), H2O2-treated PC12 (D) whole cell lysates using GTX32191 GSK3 alpha (phospho Ser21) antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399