GSK3 alpha (phospho Tyr279) + GSK3 beta (phospho Tyr216) antibody

Cat. No. GTX50316

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399)

See all GSK3 alpha products

Datasheet
Document

View product citations for antibody GTX50316 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:1000
ICC/IF	1:100-1:200
IHC-P	1:50-1:100

Not tested in other applications.

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 279/216 (V-S-Y(p)-I-C) derived from human GSK3 alpha/GSK3 beta.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycogen synthase kinase 3 alpha , glycogen synthase kinase 3 beta

Cellular Localization

Cytoplasm , Nucleus , Cell membrane

Database

Gene ID: 2931 GSK3A
Gene ID: 2932 GSK3B
UniProt: P49840 GSK3A
UniProt: P49841 GSK3B

Research Area

DATA IMAGES

GTX50316 ICC/IF Image

ICC/IF analysis of methanol-fixed MEF cells using GTX50316 GSK3 alpha (phospho Tyr279) + GSK3 beta (phospho Tyr216) antibody.

GTX50316 WB Image

WB analysis of extracts from 293 cells untreated or treated with PMA using GTX50316 GSK3 alpha (phospho Tyr279) + GSK3 beta (phospho Tyr216) antibody.

GTX50316 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50316 GSK3 alpha (phospho Tyr279) + GSK3 beta (phospho Tyr216) antibody.

GTX50316 WB Image

WB analysis of extracts from various cell lines using GTX50316 GSK3 alpha (phospho Tyr279) + GSK3 beta (phospho Tyr216) antibody.

GTX50316 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50316 GSK3 alpha (phospho Tyr279) + GSK3 beta (phospho Tyr216) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty

GSK3 alpha (phospho Tyr279) + GSK3 beta (phospho Tyr216) antibody

Cat. No. GTX50316

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Database

Research Area

DATA IMAGES

GTX50316 ICC/IF Image

GTX50316 WB Image

GTX50316 ICC/IF Image

GTX50316 WB Image

GTX50316 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.