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GSK3 alpha (phospho Tyr279) + GSK3 beta (phospho Tyr216) antibody

Anti-GSK3 alpha (phospho Tyr279) + GSK3 beta (phospho Tyr216) antibody used in IHC (Paraffin sections) (IHC-P). GTX79063
Anti-GSK3 alpha (phospho Tyr279) + GSK3 beta (phospho Tyr216) antibody used in Western Blot (WB). GTX79063

Cat. No. GTX79063

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all GSK3 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human GSK3β around the phosphorylation site of tyrosine 216 (V-S-Yp-I-C).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11168355

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycogen synthase kinase 3 alpha , glycogen synthase kinase 3 beta

Cellular Localization

Cytoplasm , Nucleus , Cell membrane

Database

Research Area

DATA IMAGES

Anti-GSK3 alpha (phospho Tyr279) + GSK3 beta (phospho Tyr216) antibody used in IHC (Paraffin sections) (IHC-P). GTX79063

GTX79063 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX79063 GSK3 alpha (phospho Tyr279) + GSK3 beta (phospho Tyr216) antibody.

Anti-GSK3 alpha (phospho Tyr279) + GSK3 beta (phospho Tyr216) antibody used in Western Blot (WB). GTX79063

GTX79063 WB Image

WB analysis of various samples using GTX79063 GSK3 alpha (phospho Tyr279) + GSK3 beta (phospho Tyr216) antibody.
Lane 1 : 293 whole cell lysate
Lane 2 : 293 whole cell lysate with 40μM insulin treatment for 30min

REFERENCE

Application Reference

2021; : e12771 Melatonin pretreatment alleviates the long-term synaptic toxicity and dysmyelination induced by neonatal Sevoflurane exposure via MT1 receptor-mediated Wnt signaling modulation.
Huang JM et al. J Nat Prod 2019; 82 (7) : 1979-1988 Moscatilin Ameliorates Tau Phosphorylation and Cognitive Deficits in Alzheimer's Disease Models.
Application : WB
Reactivity : Human
Submit a Reference

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Package List Price ($)
$ 399