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GSK3 beta antibody [1F7]

Anti-GSK3 beta antibody [1F7] used in Western Blot (WB). GTX57548
Anti-GSK3 beta antibody [1F7] used in Flow cytometry (FACS). GTX57548
Anti-GSK3 beta antibody [1F7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57548

Cat. No. GTX57548

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1F7

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($399)
See all GSK3 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:3000
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone 1F7 is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GSk5beta protein.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycogen synthase kinase 3 beta , GSK3B , GSK3 beta

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-GSK3 beta antibody [1F7] used in Western Blot (WB). GTX57548

GTX57548 WB Image

WB analysis of HeLa whole cell lysate using GTX57548 GSK3 beta antibody.
Loading : 30 μg
Dilution : 1:2000

Anti-GSK3 beta antibody [1F7] used in Flow cytometry (FACS). GTX57548

GTX57548 FACS Image

FACS analysis of HeLa cells using GTX57548 GSK3 beta antibody.
Cell Number: 1 x 10⁶ cells
Upper panel : negative control, Lower panel: Primary antibody
Antibody amount: 2-5 μg

Anti-GSK3 beta antibody [1F7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57548

GTX57548 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57548 GSK3 beta antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399