GSK3 beta antibody [4D2]

Cat No. GTX33982

Host	Mouse
Clonality	Monoclonal
Clone Name	4D2
Isotype	IgG1
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat

See all GSK3 beta products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Specificity/Sensitivity

GSK3β Mouse Monoclonal Antibody (4D2) detects endogenous levels of GSK3B

PROPERTIES

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic Peptide of GSK3β

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Glycogen Synthase Kinase 3 Beta,Gsk3B

Background

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Database

Gene ID: 2932
UniProt: P49841

Research Area

DATA IMAGES

GTX33982 IHC-P Image

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Breast Carcinoma Tissue using GSK3beta Mouse Monoclonal Antibody diluted at 1:200.

GTX33982 WB Image

Western Blot (WB) analysis of 1) HeLa Cell Lysate, 2) 3T3 Cell Lysate, 3) Rat Brain Tissue Lysate using GSK3beta Mouse Monoclonal Antibody diluted at 1:1000.