GeneTex
  • Country / Location Selection

United States (US)

GSK3 beta antibody [C1C3]

Anti-GSK3 beta antibody [C1C3] used in Western Blot (WB). GTX111192
Anti-GSK3 beta antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111192
Anti-GSK3 beta antibody [C1C3] used in Western Blot (WB). GTX111192
Anti-GSK3 beta antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111192
Anti-GSK3 beta antibody [C1C3] used in Western Blot (WB). GTX111192
Anti-GSK3 beta antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111192

Cat No. GTX111192

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000

Note :

WB
The use of GTX111192 for rat is based on IHC-P testing.

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , K562 , Mouse brain

Predict Reactivity

Zebrafish, Sheep, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GSK3 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glycogen synthase kinase 3 beta

Background

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase (see GYS1, MIM 138570). Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-GSK3 beta antibody [C1C3] used in Western Blot (WB). GTX111192

GTX111192 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSK3 beta antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111192

GTX111192 ICC/IF Image

GSK3 beta antibody [C1C3] detects GSK3 beta protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSK3 beta protein stained by GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:400.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-GSK3 beta antibody [C1C3] used in Western Blot (WB). GTX111192

GTX111192 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSK3 beta antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111192

GTX111192 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using GSK3 beta(GTX111192) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GSK3 beta antibody [C1C3] used in Western Blot (WB). GTX111192

GTX111192 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX111192 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSK3 beta antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111192

GTX111192 IHC-P Image

GSK3 beta antibody [C1C3] detects GSK3 beta protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW
There are currently no reviews for GSK3 beta antibody [C1C3] (GTX111192). Be the first to share your experience with this product.
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169