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GSK3 beta antibody [C1C3]

Anti-GSK3 beta antibody [C1C3] used in Western Blot (WB). GTX111192
Anti-GSK3 beta antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111192
Anti-GSK3 beta antibody [C1C3] used in Western Blot (WB). GTX111192
Anti-GSK3 beta antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111192
Anti-GSK3 beta antibody [C1C3] used in Western Blot (WB). GTX111192
Anti-GSK3 beta antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111192

Cat No. GTX111192

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000

Note :

WB
The use of GTX111192 for rat is based on IHC-P testing.

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , K562 , Mouse brain

Predict Reactivity

Zebrafish, Sheep, Bovine, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GSK3 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950461

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glycogen synthase kinase 3 beta

Background

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase (see GYS1, MIM 138570). Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-GSK3 beta antibody [C1C3] used in Western Blot (WB). GTX111192

GTX111192 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSK3 beta antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111192

GTX111192 ICC/IF Image

GSK3 beta antibody [C1C3] detects GSK3 beta protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSK3 beta protein stained by GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:400.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-GSK3 beta antibody [C1C3] used in Western Blot (WB). GTX111192

GTX111192 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSK3 beta antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111192

GTX111192 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using GSK3 beta(GTX111192) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GSK3 beta antibody [C1C3] used in Western Blot (WB). GTX111192

GTX111192 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX111192 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSK3 beta antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111192

GTX111192 IHC-P Image

GSK3 beta antibody [C1C3] detects GSK3 beta protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf