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GSK3 beta antibody [GT16711]

KO/KD
Anti-GSK3 beta antibody [GT16711] used in IHC (Paraffin sections) (IHC-P). GTX635816
Anti-GSK3 beta antibody [GT16711] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635816
Anti-GSK3 beta antibody [GT16711] used in Western Blot (WB). GTX635816
Anti-GSK3 beta antibody [GT16711] used in IHC (Paraffin sections) (IHC-P). GTX635816
Anti-GSK3 beta antibody [GT16711] used in Western Blot (WB). GTX635816

Cat. No. GTX635816

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT16711

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GSK3 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GSK3 beta. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888582

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycogen synthase kinase 3 beta

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-GSK3 beta antibody [GT16711] used in IHC (Paraffin sections) (IHC-P). GTX635816

GTX635816 IHC-P Image

GSK3 beta antibody [GT16711] detects GSK3 beta protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
GSK3 beta stained by GSK3 beta antibody [GT16711] (GTX635816) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GSK3 beta antibody [GT16711] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635816

GTX635816 ICC/IF Image

GSK3 beta antibody detects GSK3 beta protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSK3 beta stained by GSK3 beta antibody (GTX635816) diluted at 1:500.

Anti-GSK3 beta antibody [GT16711] used in Western Blot (WB). GTX635816

GTX635816 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [GT16711] (GTX635816) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GSK3 beta antibody [GT16711] used in IHC (Paraffin sections) (IHC-P). GTX635816

GTX635816 IHC-P Image

GSK3 beta antibody [GT16711] detects GSK3 beta protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
GSK3 beta stained by GSK3 beta antibody [GT16711] (GTX635816) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GSK3 beta antibody [GT16711] used in Western Blot (WB). GTX635816

GTX635816 WB Image

KO/KD

Wild-type (WT) and GSK3 beta knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [GT16711] (GTX635816) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Liu Y.J. et al. Int. J. Mol. Sci. 2021; 22 (6) : 2823 The Anti-Melanogenesis Effect of 3,4-Dihydroxybenzalacetone through Downregulation of Melanosome Maturation and Transportation in B16F10 and Human Epidermal Melanocytes.
Application : WB
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REVIEW

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Package List Price ($)
$ 399
$ 169