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GSK3 beta antibody [GT16711]

KO/KD
Anti-GSK3 beta antibody [GT16711] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635816
Anti-GSK3 beta antibody [GT16711] used in Western Blot (WB). GTX635816
Anti-GSK3 beta antibody [GT16711] used in Western Blot (WB). GTX635816
Anti-GSK3 beta antibody [GT16711] used in IHC (Paraffin sections) (IHC-P). GTX635816
Anti-GSK3 beta antibody [GT16711] used in IHC (Paraffin sections) (IHC-P). GTX635816

Cat No. GTX635816

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT16711

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all GSK3 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GSK3 beta. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888582

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glycogen synthase kinase 3 beta

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-GSK3 beta antibody [GT16711] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635816

GTX635816 ICC/IF Image

GSK3 beta antibody detects GSK3 beta protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSK3 beta stained by GSK3 beta antibody (GTX635816) diluted at 1:500.

Anti-GSK3 beta antibody [GT16711] used in Western Blot (WB). GTX635816

GTX635816 WB Image

KO/KD

Wild-type (WT) and GSK3 beta knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [GT16711] (GTX635816) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GSK3 beta antibody [GT16711] used in Western Blot (WB). GTX635816

GTX635816 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [GT16711] (GTX635816) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GSK3 beta antibody [GT16711] used in IHC (Paraffin sections) (IHC-P). GTX635816

GTX635816 IHC-P Image

GSK3 beta antibody [GT16711] detects GSK3 beta protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
GSK3 beta stained by GSK3 beta antibody [GT16711] (GTX635816) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GSK3 beta antibody [GT16711] used in IHC (Paraffin sections) (IHC-P). GTX635816

GTX635816 IHC-P Image

GSK3 beta antibody [GT16711] detects GSK3 beta protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
GSK3 beta stained by GSK3 beta antibody [GT16711] (GTX635816) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Liu Y.J. et al. Int. J. Mol. Sci. 2021; 22 (6) : 2823 The Anti-Melanogenesis Effect of 3,4-Dihydroxybenzalacetone through Downregulation of Melanosome Maturation and Transportation in B16F10 and Human Epidermal Melanocytes.
Application : WB
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Package List Price ($)
$ 329
$ 169