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GSK3 beta antibody

Anti-GSK3 beta antibody used in Western Blot (WB). GTX133372
Anti-GSK3 beta antibody used in Western Blot (WB). GTX133372
Anti-GSK3 beta antibody used in Western Blot (WB). GTX133372
Anti-GSK3 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133372

Cat No. GTX133372

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , A431 , HeLa

Product Note

Based on sequence homology, this antibody is predicted to recognize both GSK3B isoforms 1 and 2.

Predict Reactivity

Sheep, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GSK3 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886960

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glycogen synthase kinase 3 beta

Background

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase (see GYS1, MIM 138570). Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-GSK3 beta antibody used in Western Blot (WB). GTX133372

GTX133372 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody (GTX133372) diluted at 1:1000.

Anti-GSK3 beta antibody used in Western Blot (WB). GTX133372

GTX133372 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody (GTX133372) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSK3 beta antibody used in Western Blot (WB). GTX133372

GTX133372 WB Image

Wild-type (WT) and GSK3 beta knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody (GTX133372) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSK3 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133372

GTX133372 ICC/IF Image

GSK3 beta antibody detects GSK3 beta protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSK3 beta stained by GSK3 beta antibody (GTX133372) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker diluted at 1:200.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169