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GSK3 beta antibody

Anti-GSK3 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX37303
Anti-GSK3 beta antibody used in Western Blot (WB). GTX37303
Anti-GSK3 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX37303

Cat. No. GTX37303

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
50 μl ($399)
See all GSK3 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized non-phosphopeptide derived from human GSK3 beta around the phosphorylation site of serine 9 (T -T-S(p)-F-A).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycogen synthase kinase 3 beta

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-GSK3 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX37303

GTX37303 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX37303 GSK3 beta antibody.

Anti-GSK3 beta antibody used in Western Blot (WB). GTX37303

GTX37303 WB Image

WB analysis of A2780 and HT-29 cell lysates using GTX37303 GSK3 beta antibody.

Anti-GSK3 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX37303

GTX37303 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX37303 GSK3 beta antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399