GeneTex
United States (US)

GSK3 beta (phospho Ser9) antibody

GTX29769_20160330_WB_18121410_810.jpg
GTX29769_20160330_IHC-P_18121410_892.jpg

Cat No. GTX29769

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Drosophila
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:3000
ICC/IF 1:200 - 1:1000
IHC-P 1:200 - 1:1000
ELISA 1:10000 - 1:50000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: RRTTpSFAE, corresponding to amino acids 4-12 of Human GSK3 beta.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glycogen Synthase Kinase 3 Beta,Gsk3B

Background

Glycogen synthase kinase-3 (GSK 3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK 3A) and beta, show a high degree of amino acid homology. GSK 3B is involved in energy metabolism, neuronal cell development, and body pattern formation.

Database

Research Area

DATA IMAGES
GTX29769_20160330_WB_18121410_810.jpg

GTX29769 WB Image

Western blot using GeneTex Immunochemical's Rabbit-anti-GSK3B pS9 antibody at a 1:1,000 dilution. All lanes contain human 293T whole cell lysate showing a band at 47 kDa. Cells were serum starved for 24 h prior to extraction. Lane 1: Control, Lane 2: treated with IGF-1 (100 ng/ml) for 20' , lane 3: pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20'. Molecular weight markers confirm a MW of ~ 49 kDa.

GTX29769_20160330_IHC-P_18121410_892.jpg

GTX29769 IHC-P Image

Rabbit anti-GSK3B pS9 (GTX29769) was used at a 1:200 dilution to detect GSK3B by immunohistochemistry in human ovarian cancer tumor tissue. Tissue was formalin-fixed and paraffin embedded.

REFERENCE
Package List Price ($)
$ 409