WB analysis of treated and untreated 293T whole cell lysate using GTX29769 GSK3 beta (phospho Ser9) antibody.
Lane 1 : Mock
Lane 2 : IGF-1 treated whole cell lysate
Lane 3 : Pre-teated with LY294002 and treated with IGF-1 whole cell lysate

Western blot using GeneTex Immunochemical's Rabbit-anti-GSK3B pS9 antibody at a 1:1,000 dilution. All lanes contain human 293T whole cell lysate showing a band at 47 kDa. Cells were serum starved for 24 h prior to extraction. Lane 1: Control, Lane 2: treated with IGF-1 (100 ng/ml) for 20' , lane 3: pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20'. Molecular weight markers confirm a MW of ~ 49 kDa.

Rabbit anti-GSK3B pS9 (GTX29769) was used at a 1:200 dilution to detect GSK3B by immunohistochemistry in human ovarian cancer tumor tissue. Tissue was formalin-fixed and paraffin embedded.