GeneTex

  • Country / Location Selection

United States (US)

GSK3 beta (phospho Ser9) antibody

Orthogonal
Anti-GSK3 beta (phospho Ser9) antibody used in IHC (Paraffin sections) (IHC-P). GTX29769
Anti-GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX29769
Anti-GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX29769

Cat. No. GTX29769

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Drosophila
Package
100 μg ($589)
See all GSK3 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:200-1:1000
IHC-P 1:200-1:1000
ELISA 1:10000-1:50000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Product Note

This phospho specific polyclonal antibody reacts with phosphorylated pS9 of human GSK3B. Reactivity with non-phosphorylated human GSK3B is minimal.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to a N-Terminal region near aa 1-15 of human GSK3 beta.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

RRID

AB_370828

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycogen synthase kinase 3 beta

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

Glycogen synthase kinase-3 (GSK 3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK 3A) and beta, show a high degree of amino acid homology. GSK 3B is involved in energy metabolism, neuronal cell development, and body pattern formation.

Database

Research Area

DATA IMAGES

Anti-GSK3 beta (phospho Ser9) antibody used in IHC (Paraffin sections) (IHC-P). GTX29769

GTX29769 IHC-P Image

Rabbit anti-GSK3B pS9 (GTX29769) was used at a 1:200 dilution to detect GSK3B by immunohistochemistry in human ovarian cancer tumor tissue. Tissue was formalin-fixed and paraffin embedded.

Anti-GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX29769

GTX29769 WB Image

Western blot using GeneTex Immunochemical's Rabbit-anti-GSK3B pS9 antibody at a 1:1,000 dilution. All lanes contain human 293T whole cell lysate showing a band at 47 kDa. Cells were serum starved for 24 h prior to extraction. Lane 1: Control, Lane 2: treated with IGF-1 (100 ng/ml) for 20' , lane 3: pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20'. Molecular weight markers confirm a MW of ~ 49 kDa.

Anti-GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX29769

GTX29769 WB Image

Orthogonal

WB analysis of treated and untreated 293T whole cell lysate using GTX29769 GSK3 beta (phospho Ser9) antibody.
Lane 1 : Mock
Lane 2 : IGF-1 treated whole cell lysate
Lane 3 : Pre-teated with LY294002 and treated with IGF-1 whole cell lysate

REFERENCE

Application Reference

Ambegaokar SS et al. Genetics 2010; 186 (1) : 435-42 Interaction between eye pigment genes and tau-induced neurodegeneration in Drosophila melanogaster.
Application : WB
Reactivity : Drosophila
Submit a Reference

REVIEW

There are currently no reviews for GSK3 beta (phospho Ser9) antibody (GTX29769). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 589