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GSK3 beta (phospho Ser9) antibody

Anti-GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX50090
Anti-GSK3 beta (phospho Ser9) antibody used in IHC (Paraffin sections) (IHC-P). GTX50090
Anti-GSK3 beta (phospho Ser9) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50090
Anti-GSK3 beta (phospho Ser9) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50090
Anti-GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX50090
Anti-GSK3 beta (phospho Ser9) antibody used in IHC (Paraffin sections) (IHC-P). GTX50090

Cat No. GTX50090

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 9 (T-T-S(p)-F-A) derived from human GSK3 beta.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glycogen synthase kinase 3 beta

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX50090

GTX50090 WB Image

WB analysis of extracts from 293 cells untreated (lane 1) or treated with serum (lane 2) using GTX50090 GSK3 beta (phospho Ser9) antibody.

Anti-GSK3 beta (phospho Ser9) antibody used in IHC (Paraffin sections) (IHC-P). GTX50090

GTX50090 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX50090 GSK3 beta (phospho Ser9) antibody.

Anti-GSK3 beta (phospho Ser9) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50090

GTX50090 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50090 GSK3 beta (phospho Ser9) antibody.

Anti-GSK3 beta (phospho Ser9) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50090

GTX50090 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50090 GSK3 beta (phospho Ser9) antibody.

Anti-GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX50090

GTX50090 WB Image

WB analysis of extracts from HeLa cells treated with EGF or calf intestinal phosphatase (CIP) using GTX50090 GSK3 beta (phospho Ser9) antibody.

Anti-GSK3 beta (phospho Ser9) antibody used in IHC (Paraffin sections) (IHC-P). GTX50090

GTX50090 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50090 GSK3 beta (phospho Ser9) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf