GeneTex

  • Country / Location Selection

United States (US)

GSK3 beta (phospho Ser9) antibody

Anti-GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX50090
Anti-GSK3 beta (phospho Ser9) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50090
Anti-GSK3 beta (phospho Ser9) antibody used in IHC (Paraffin sections) (IHC-P). GTX50090
Anti-GSK3 beta (phospho Ser9) antibody used in IHC (Paraffin sections) (IHC-P). GTX50090
Anti-GSK3 beta (phospho Ser9) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50090
Anti-GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX50090

Cat. No. GTX50090

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all GSK3 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 9 (T-T-S(p)-F-A) derived from human GSK3 beta.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11176436

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycogen synthase kinase 3 beta

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX50090

GTX50090 WB Image

WB analysis of extracts from 293 cells untreated (lane 1) or treated with serum (lane 2) using GTX50090 GSK3 beta (phospho Ser9) antibody.

Anti-GSK3 beta (phospho Ser9) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50090

GTX50090 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50090 GSK3 beta (phospho Ser9) antibody.

Anti-GSK3 beta (phospho Ser9) antibody used in IHC (Paraffin sections) (IHC-P). GTX50090

GTX50090 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX50090 GSK3 beta (phospho Ser9) antibody.

Anti-GSK3 beta (phospho Ser9) antibody used in IHC (Paraffin sections) (IHC-P). GTX50090

GTX50090 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50090 GSK3 beta (phospho Ser9) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-GSK3 beta (phospho Ser9) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50090

GTX50090 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50090 GSK3 beta (phospho Ser9) antibody.

Anti-GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX50090

GTX50090 WB Image

WB analysis of extracts from HeLa cells treated with EGF or calf intestinal phosphatase (CIP) using GTX50090 GSK3 beta (phospho Ser9) antibody.

REFERENCE

Application Reference

Jadhao M et al. Antioxidants (Basel) 2021; 10 (6) The Long-Term DEHP Exposure Confers Multidrug Resistance of Triple-Negative Breast Cancer Cells through ABC Transporters and Intracellular ROS.
Application : WB
Reactivity : Human
Chang YX et al. Cancers (Basel) 2020; 12 (12) Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways.
Application : WB
Reactivity : Human
Pan CH et al. Chem Biol Interact 2020; 332 : 109304
Application : WB
Reactivity : Human
Zhang XL et al. Oncotarget 2017; 8 (57) : 96809-96825 Pnma5 is essential to the progression of meiosis in mouse oocytes through a chain of phosphorylation.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for GSK3 beta (phospho Ser9) antibody (GTX50090). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399