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GSPT1 antibody

Anti-GSPT1 antibody used in Western Blot (WB). GTX105475
Anti-GSPT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105475
Anti-GSPT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105475
Anti-GSPT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105475
Anti-GSPT1 antibody used in Western Blot (WB). GTX105475
Anti-GSPT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105475

Cat. No. GTX105475

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GSPT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173323

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G1 to S phase transition 1 , 551G9.2 , ETF3A , GST1 , eRF3a

Database

Research Area

DATA IMAGES

Anti-GSPT1 antibody used in Western Blot (WB). GTX105475

GTX105475 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody (GTX105475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSPT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105475

GTX105475 IHC-P Image

GSPT1 antibody detects GSPT1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
GSPT1 stained by GSPT1 antibody (GTX105475) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GSPT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105475

GTX105475 IHC-P Image

GSPT1 antibody detects GSPT1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
GSPT1 stained by GSPT1 antibody (GTX105475) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GSPT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105475

GTX105475 ICC/IF Image

GSPT1 antibody detects GSPT1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSPT1 stained by GSPT1 antibody (GTX105475) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GSPT1 antibody used in Western Blot (WB). GTX105475

GTX105475 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody (GTX105475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSPT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105475

GTX105475 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using GSPT1(GTX105475) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169