GeneTex
United States (US)

GSPT1 antibody

GTX105475_42235_20151008_WB_19011506_358.jpg
GTX105475_40618_IHC_19011506_961.jpg
GTX105475_42235_20151223_IFA_19011506_304.jpg

Cat No. GTX105475

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GSPT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

551G9.2 antibody, ETF3A antibody, FLJ38048 antibody, FLJ39067 antibody, GST1 antibody, eRF3a antibody, GSPT1 antibody, eukaryotic peptide chain release factor subunit 3a antibody, eukaryotic peptide chain release factor GTP-binding subunit ERF3A antibody, G1 to S phase transition protein 1 homolog antibody, G1 to S phase transition 1 antibody

Database

Research Area

DATA IMAGES
GTX105475_42235_20151008_WB_19011506_358.jpg

GTX105475 WB Image

GSPT1 antibody detects GSPT1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody (GTX105475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105475_40618_IHC_19011506_961.jpg

GTX105475 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using GSPT1(GTX105475) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105475_42235_20151223_IFA_19011506_304.jpg

GTX105475 ICC/IF Image

GSPT1 antibody detects GSPT protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSPT protein stained by GSPT1 antibody (GTX105475) diluted at 1:500.
Blue: Hoechst 33342 staining.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169