Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody (GTX105475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using GSPT1(GTX105475) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody (GTX105475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GSPT1 antibody detects GSPT1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSPT1 stained by GSPT1 antibody (GTX105475) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GSPT1 antibody detects GSPT1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
GSPT1 stained by GSPT1 antibody (GTX105475) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GSPT1 antibody detects GSPT1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
GSPT1 stained by GSPT1 antibody (GTX105475) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min