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GST tag antibody [3G10]

Anti-GST tag antibody [3G10] used in Western Blot (WB). GTX70195
Anti-GST tag antibody [3G10] used in Western Blot (WB). GTX70195

Cat. No. GTX70195

Reference 6

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3G10

Isotype

 IgG1

Application

 WB, IP
Package
100 μl ($399)
See all GST tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1 - 2 μg/ml
IP Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

GST protein of the parasitic helminth Schistosoma japonicum expressed in E. coli

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_371442

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

GST tag

Research Area

DATA IMAGES

Anti-GST tag antibody [3G10] used in Western Blot (WB). GTX70195

GTX70195 WB Image

GST tag antibody [3G10] detects GST tag protein by Western blot analysis.
A. Non-induced cell lysate from E. coli expressing GST-tagged proteins
B. IPTG-induced cell lysate from E. coli expressing GST-tagged proteins
10 % SDS-PAGE
GST tag antibody [3G10] (GTX70195) dilution: 1:10000

Anti-GST tag antibody [3G10] used in Western Blot (WB). GTX70195

GTX70195 WB Image

GST tag antibody [3G10] detects GST tag protein by Western blot analysis.
A. 2 μg His-recombinant protein
B. 2 μg GST-recombinant protein
C. 1 μg GST-recombinant protein
D. 0.5 μg GST-recombinant protein
10 % SDS-PAGE
GST tag antibody [3G10] (GTX70195) dilution: 1:5000

REFERENCE

Application Reference

Takeharu Sakamoto et al. Commun Biol 2021; 4 (1) : 1165 Pharmacological inhibition of Mint3 attenuates tumour growth, metastasis, and endotoxic shock.
Application : WB
Morohoshi K et al. J Reprod Immunol 2021; 148 : 103436 Identification of an antibacterial polypeptide in mouse seminal vesicle secretions.
Afolayan AJ et al. J Biol Chem 2016; (Epub) Domain Mapping of the Heat Shock Protein 70 reveals that Glutamic acid 446 and Arginine 447 are Critical for Regulating Superoxide Dismutase-2 Function.
Application : IP
Koppen-Rung P et al. J Virol 2016; (Epub) Intracellular distributions of capsid-associated pUL77 of HCMV and interactions with packaging proteins and pUL93.
Application : IP
Siepe D et al. Nat Cell Biol 2009; 11 (8) : 967-72 Prolyl isomerase Pin1 acts as a switch to control the degree of substrate ubiquitylation.
Application : IP
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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations