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GST tag antibody [8-826]

Anti-GST tag antibody [8-826] used in Immunoprecipitation (IP). GTX79438
Anti-GST tag antibody [8-826] used in Western Blot (WB). GTX79438

Cat No. GTX79438

Host

Mouse

Clonality

Monoclonal

Clone Name

8-826

Isotype

IgG

Application

WB, ICC/IF, IP, ELISA
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF Assay dependent
IP 2 μg
ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant purified full-length GST

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-GST tag antibody [8-826] used in Immunoprecipitation (IP). GTX79438

GTX79438 IP Image

Immuno-precipitation of GST-Shp2 SH2 domain (Lane 2, 39kDa) was performed by mixing 2μg of purified fusion protein with 1μg of GST antibody [8-326] overnight on a rocking platform at 4ºC. The immune-complex was then captured on 50μl Protein A/G Agarose. Captured immune-complexes were then washed extensively and proteins eluted. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. IP efficiency was compared to a non-IP loading control consisting of 1ug GST-Shp2 SH2 domain (Lane1). Proteins were transferred to PVDF membrane and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with GST antibody [8-326] at a dilution of 1:1000 overnight rotating at 4ºC. Membranes were then washed in TBS/0.1% Tween-20 and probed with a proper secondary antibody. Membranes were washed and chemiluminescent detection was performed.

Anti-GST tag antibody [8-826] used in Western Blot (WB). GTX79438

GTX79438 WB Image

Western blot analysis of GST-Abl SH2 domain (Lane 1, 35kDa) and GST-Grb2 SH2 domain (Lane 2, 38kDa). Proteins were transferred to a PVDF membrane and blocked with 5% BSA/0.1%TBST for at least 1 hour. Membranes were then probed with GST antibody [8-326] at a dilution of 1:1000 overnight at 4ºC on a rocking platform. Membranes were then washed in TBS/0.1%Tween-20 and probed with a HRP-conjugated secondary antibody. Membranes were washed and chemiluminescent detection was performed.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339