GST tag antibody [8-826]

Cat. No. GTX79438

Host	Mouse
Clonality	Monoclonal
Clone Name	8-826
Isotype	IgG
Application	WB, ICC/IF, IP, ELISA
Package	100 μl ($399)

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Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000
ICC/IF	Assay dependent
IP	2 μg
ELISA	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant purified full-length GST

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_11178764

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

GTX79438 IP Image

Immuno-precipitation of GST-Shp2 SH2 domain (Lane 2, 39kDa) was performed by mixing 2μg of purified fusion protein with 1μg of GST antibody [8-326] overnight on a rocking platform at 4ºC. The immune-complex was then captured on 50μl Protein A/G Agarose. Captured immune-complexes were then washed extensively and proteins eluted. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. IP efficiency was compared to a non-IP loading control consisting of 1ug GST-Shp2 SH2 domain (Lane1). Proteins were transferred to PVDF membrane and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with GST antibody [8-326] at a dilution of 1:1000 overnight rotating at 4ºC. Membranes were then washed in TBS/0.1% Tween-20 and probed with a proper secondary antibody. Membranes were washed and chemiluminescent detection was performed.

GTX79438 WB Image

Western blot analysis of GST-Abl SH2 domain (Lane 1, 35kDa) and GST-Grb2 SH2 domain (Lane 2, 38kDa). Proteins were transferred to a PVDF membrane and blocked with 5% BSA/0.1%TBST for at least 1 hour. Membranes were then probed with GST antibody [8-326] at a dilution of 1:1000 overnight at 4ºC on a rocking platform. Membranes were then washed in TBS/0.1%Tween-20 and probed with a HRP-conjugated secondary antibody. Membranes were washed and chemiluminescent detection was performed.

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty

GST tag antibody [8-826]

Cat. No. GTX79438

Host

Clonality

Clone Name

Isotype

Application