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GST tag antibody (Biotin)

Cat No. GTX26635

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA, IHC
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000 - 1:10000
ELISA 1:10000 - 1:50000
IHC 1:1000 - 1:5000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate pH7.2, 0.15M Sodium Chloride, 10mg/ml BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Glutathione-S-Transferase [Schistosoma japonicum]

Purification

Purified by antigen-affinity chromatography.
IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.

Conjugation

Biotin
Ratio : 10-20 Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) molecules per Goat IgG molecule.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

This highly active polyclonal antibody is directed against purified recombinant Glutathione-S-Transferase (GST) cloned from the organism Schistosoma japonicum. This reagent is specifically designed for screening and recovery of fusion proteins from expression systems using a GST gene cloning site

Research Area

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 379