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GST tag antibody (HRP)

Anti-GST tag antibody (HRP) used in Western Blot (WB). GTX114099

Cat. No. GTX114099

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all GST tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

TBS, 1% BSA

Preservative

0.005% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to GST

Purification

Purified by antigen-affinity chromatography.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_1949436

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GST-HRP , GSTHRP , GST HRP

Research Area

DATA IMAGES

Anti-GST tag antibody (HRP) used in Western Blot (WB). GTX114099

GTX114099 WB Image

Sample
A: BSA (20 ng)
B: GST (20 ng)
C: GST (10 ng)
D: GST (1 ng)
12% SDS PAGE
GTX114099 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wang J et al. Nucleic Acids Res 2022; 50 (19) : 10929-10946 A cryptic transactivation domain of EZH2 binds AR and AR's splice variant, promoting oncogene activation and tumorous transformation.
Katarzyna Z Haza et al. Nat Commun 2021; 12 (1) : 4045 RAS-inhibiting biologics identify and probe druggable pockets including an SII-α3 allosteric site.
Application : WB
Enrico TP et al. Elife 2021; 10 Cyclin F drives proliferation through SCF-dependent degradation of the retinoblastoma-like tumor suppressor p130/RBL2.
Fan H et al. Nat Genet 2020; 52 (12) : 1384-1396 BAHCC1 binds H3K27me3 via a conserved BAH module to mediate gene silencing and oncogenesis.
Chen TI et al. J Virol 2019; 93 (22) Hepatitis C Virus NS3 Protein Plays a Dual Role in WRN-Mediated Repair of Nonhomologous End Joining.
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REVIEW

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SDS
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169