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GST tag antibody (HRP)

Anti-GST tag antibody (HRP) used in Western Blot (WB). GTX114099

Cat. No. GTX114099

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB
Package
100 μl ($399),
25 μl ($169)
See all GST tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

TBS pH7.4, 10mg/ml BSA

Preservative

0.005% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.178 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to GST

Purification

Purified by antigen-affinity chromatography.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_1949436

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

GST-HRP , GSTHRP , GST HRP

Research Area

DATA IMAGES

Anti-GST tag antibody (HRP) used in Western Blot (WB). GTX114099

GTX114099 WB Image

Sample
A: BSA (20 ng)
B: GST (20 ng)
C: GST (10 ng)
D: GST (1 ng)
12% SDS PAGE
GTX114099 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wang J et al. Nucleic Acids Res 2022; 50 (19) : 10929-10946 A cryptic transactivation domain of EZH2 binds AR and AR's splice variant, promoting oncogene activation and tumorous transformation.
Katarzyna Z Haza et al. Nat Commun 2021; 12 (1) : 4045 RAS-inhibiting biologics identify and probe druggable pockets including an SII-α3 allosteric site.
Application : WB
Enrico TP et al. Elife 2021; 10 Cyclin F drives proliferation through SCF-dependent degradation of the retinoblastoma-like tumor suppressor p130/RBL2.
Fan H et al. Nat Genet 2020; 52 (12) : 1384-1396 BAHCC1 binds H3K27me3 via a conserved BAH module to mediate gene silencing and oncogenesis.
Chen TI et al. J Virol 2019; 93 (22) Hepatitis C Virus NS3 Protein Plays a Dual Role in WRN-Mediated Repair of Nonhomologous End Joining.
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REVIEW

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SDS
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169