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GST tag antibody

Anti-GST tag antibody used in Immunoprecipitation (IP). GTX110736
Anti-GST tag antibody used in Western Blot (WB). GTX110736

Cat No. GTX110736

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, ELISA

Reactivity

Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.06 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to GST

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-GST tag antibody used in Immunoprecipitation (IP). GTX110736

GTX110736 IP Image

GST-tagged protein (Mw: 45 kDa) was immunoprecipitated from E. coli lysate using 8 ug of anti-GST antibody (GTX110736).
The precipitated protein was detected by GTX110736 diluted at 1:3000.

Anti-GST tag antibody used in Western Blot (WB). GTX110736

GTX110736 WB Image

Sample
A: 20μg of E. coli only
B: 20μg of Transformed E. coli
C: 20μg of Transformed E. coli and IPTG induced12% SDS PAGE
GTX110736 diluated at 1:3000

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169