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GST tag antibody

Anti-GST tag antibody used in Immunoprecipitation (IP). GTX110736
Anti-GST tag antibody used in Western Blot (WB). GTX110736

Cat No. GTX110736

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, ELISA
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7), 1% BSA. 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to GST

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949427

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-GST tag antibody used in Immunoprecipitation (IP). GTX110736

GTX110736 IP Image

GST-tagged protein (Mw: 45 kDa) was immunoprecipitated from E. coli lysate using 8 ug of anti-GST antibody (GTX110736).
The precipitated protein was detected by GTX110736 diluted at 1:3000.

Anti-GST tag antibody used in Western Blot (WB). GTX110736

GTX110736 WB Image

IPTG-induced cell lysate from E. coli expressing GST-tag tagged proteins were separated by 10% SDS-PAGE, and the membrane was blotted with GST tag antibody (GTX110736) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

GST tag antibody Cat No. GTX110736

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal BMC Biol in 2017.PMID: 29183317
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Elife in 2018.PMID: 30516470
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169