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GST tag antibody

Anti-GST tag antibody used in Western Blot (WB). GTX82896

Cat No. GTX82896

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:5000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

GST-tagged fusion proteins.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAGR, green fluorescent protein (GFP), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4. FLAGR and anti-FLAGR are registered trademarks of Sigma-Aldrich Biotechnology Co.

Research Area

DATA IMAGES

Anti-GST tag antibody used in Western Blot (WB). GTX82896

GTX82896 WB Image

WB analysis of 25 ug of 293T cells transfected with GST-MART1 (Lane 1), HepG2 cells (Lane 2) and 293T cells transfected with 12 Epitope Tag (12Tag) (Lane 3) using GTX82896 GST Epitope Tag antibody.
Dilution : 1:5000 (GST and 12Tag) and 1:1000 (HepG2)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379