APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:5000 |
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
PBS, 1mg/ml BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
GST-tagged fusion proteins.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Background
Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAGR, green fluorescent protein (GFP), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4. FLAGR and anti-FLAGR are registered trademarks of Sigma-Aldrich Biotechnology Co.
Research Area
DATA IMAGES
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GTX82896 WB Image
WB analysis of 25 ug of 293T cells transfected with GST-MART1 (Lane 1), HepG2 cells (Lane 2) and 293T cells transfected with 12 Epitope Tag (12Tag) (Lane 3) using GTX82896 GST Epitope Tag antibody. Dilution : 1:5000 (GST and 12Tag) and 1:1000 (HepG2)
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REFERENCE
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REVIEW
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