GeneTex
United States (US)

GSTA1 antibody

GTX108012_39855_IHC_19011507_180.jpg
GTX108012_WB_19011507_593.jpg
GTX108012_39855_IFA_19011507_816.jpg
GTX108012_39855_WB_M_liver_19011507_531.jpg

Cat No. GTX108012

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

HepG2 , mouse liver
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein corresponding to human GSTA1

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glutathione S-Transferase Alpha 1,Gst-Epsilon,Gst2,Gsta1-1,Gth1,Gsta1

Cellular Localization

Cytoplasm

Background

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver. In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity thereby protecting the cells from reactive oxygen species and the products of peroxidation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108012_39855_IHC_19011507_180.jpg

GTX108012 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using GSTA1(GTX108012) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108012_WB_19011507_593.jpg

GTX108012 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GSTA1 antibody
GTX108012 diluted at 1:1000

GTX108012_39855_IFA_19011507_816.jpg

GTX108012 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep3B, using GSTA1(GTX108012) antibody at 1:500 dilution.

GTX108012_39855_WB_M_liver_19011507_531.jpg

GTX108012 WB Image

GSTA1 antibody detects GSTA1 protein by Western blot analysis.
A. 50 µg mouse liver lysate/extract
12 % SDS-PAGE
GSTA1 antibody (GTX108012) dilution: 1:5000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169