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GSTA2 antibody [N1C3]

Anti-GSTA2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105600
Anti-GSTA2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105600
Anti-GSTA2 antibody [N1C3] used in Western Blot (WB). GTX105600

Cat No. GTX105600

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GSTA2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glutathione S-transferase alpha 2 , GST2 , GSTA2-2 , GTA2 , GTH2

Cellular Localization

Cytoplasm

Background

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver. In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity thereby protecting the cells from reactive oxygen species and the products of peroxidation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-GSTA2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105600

GTX105600 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep G2, using GSTA2(GTX105600) antibody at 1:500 dilution.

Anti-GSTA2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105600

GTX105600 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human stomach, using GSTA2(GTX105600) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GSTA2 antibody [N1C3] used in Western Blot (WB). GTX105600

GTX105600 WB Image

Sample(30 µg of whole cell lysate)
A:Hep G2(GTX27900)
12% SDS PAGE
GTX105600 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf