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GSTA4 antibody [N1C3]

Anti-GSTA4 antibody [N1C3] used in Western Blot (WB). GTX104390
Anti-GSTA4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104390

Cat. No. GTX104390

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Pig
Package
100 μl ($399),
25 μl ($169)
See all GSTA4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GSTA4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950467

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutathione S-transferase alpha 4 , GSTA4-4 , GTA4

Cellular Localization

Cytoplasm

Background

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, which are located in a cluster on chromosome 6, are highly related and encode enzymes with glutathione peroxidase activity that function in the detoxification of lipid peroxidation products. Reactive electrophiles produced by oxidative metabolism have been linked to a number of degenerative diseases including Parkinson's disease, Alzheimer's disease, cataract formation, and atherosclerosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GSTA4 antibody [N1C3] used in Western Blot (WB). GTX104390

GTX104390 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GST A4 antibody
GTX104390 diluted at 1:1000

Anti-GSTA4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104390

GTX104390 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using GSTA4(GTX104390) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Weber TE et al. Livestock Science. 2011; 142 : 85-91 Effect of dietary distillers dried grains with solubles on indicators of oxidative stress and immune function in growing pigs.
Application : WB
Reactivity : Pig
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169