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GSTM1 antibody

Orthogonal
Anti-GSTM1 antibody used in Western Blot (WB). GTX113448
Anti-GSTM1 antibody used in Western Blot (WB). GTX113448
Anti-GSTM1 antibody used in Western Blot (WB). GTX113448
Anti-GSTM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113448
Anti-GSTM1 antibody used in Western Blot (WB). GTX113448
Anti-GSTM1 antibody used in Western Blot (WB). GTX113448

Cat. No. GTX113448

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GSTM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

rat liver , mouse brain , human liver

Predict Reactivity

Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GSTM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037100

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutathione S-transferase mu 1 , GST1 , GSTM1-1 , GSTM1a-1a , GSTM1b-1b , GTH4 , GTM1 , H-B , MU , MU-1

Cellular Localization

Cytoplasm

Background

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Null mutations of this class mu gene have been linked with an increase in a number of cancers, likely due to an increased susceptibility to environmental toxins and carcinogens. Multiple protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GSTM1 antibody used in Western Blot (WB). GTX113448

GTX113448 WB Image

GSTM1 antibody detects protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
12% SDS-PAGE
GSTM1 antibody (GTX113448) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSTM1 antibody used in Western Blot (WB). GTX113448

GTX113448 WB Image

GSTM1 antibody detects GSTM1 protein by western blot analysis.
A. 50 μg rat liver lysate/extract
12% SDS-PAGE
GSTM1 antibody (GTX113448) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSTM1 antibody used in Western Blot (WB). GTX113448

GTX113448 WB Image

Human tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with GSTM1 antibody (GTX113448) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSTM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113448

GTX113448 IHC-P Image

GSTM1 antibody detects GSTM1 protein at cytoplasm in rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
GSTM1 antibody (GTX113448) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GSTM1 antibody used in Western Blot (WB). GTX113448

GTX113448 WB Image

Orthogonal

The data was published in the journal PLoS One in 2016.PMID: 27404495

Anti-GSTM1 antibody used in Western Blot (WB). GTX113448

GTX113448 WB Image

The data was published in the journal PLoS One in 2017. PMID: 29016672

REFERENCE

Application Reference

Zamami Y et al. Biomed Pharmacother 2022; 148 : 112744
Application : WB
Reactivity : Rat
Dai YC et al. PLoS One 2017; 12 (10) : e0186214 The interaction of arsenic and N-butyl-N-(4-hydroxybutyl)nitrosamine on urothelial carcinogenesis in mice.
Application : WB
Reactivity : Mouse
Wang SC et al. PLoS One 2016; 11 (7) : e0159102 Gene Expression and DNA Methylation Status of Glutathione S-Transferase Mu1 and Mu5 in Urothelial Carcinoma.
Application : WB
Reactivity : Mouse
Gdula-Argasi?ska J et al. Pharmacol Rep 2016; 68 (5) : 939-944 Resolvin D1 down-regulates CYP1A1 and PTGS2 gene in the HUVEC cells treated with benzo(a)pyrene.
Application : WB
Reactivity : Human
Chuang JJ et al. Toxicol Appl Pharmacol 2014; Downregulation of glutathione S-transferase * protein in N-butyl-N-(4-hydroxybutyl)nitrosamine-induced mouse bladder carcinogenesis.
Application : WB
Reactivity : Mouse
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169