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GSTO1 antibody [N1C3]

Anti-GSTO1 antibody [N1C3] used in Western Blot (WB). GTX105655
Anti-GSTO1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105655
Anti-GSTO1 antibody [N1C3] used in Western Blot (WB). GTX105655
Anti-GSTO1 antibody [N1C3] used in Western Blot (WB). GTX105655

Cat No. GTX105655

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , mouse colon

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GSTO1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glutathione S-Transferase Omega 1,Gsto 1-1,Gsttlp28,Hel-S-21,P28,Spg-R,Gsto1

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the theta class glutathione S-transferase-like (GSTTL) protein family. In mouse, the encoded protein acts as a small stress response protein, likely involved in cellular redox homeostasis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-GSTO1 antibody [N1C3] used in Western Blot (WB). GTX105655

GTX105655 WB Image

Wild-type (WT) and GSTO1 knockout (KO) 293T cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with GSTO1 antibody [N1C3] (GTX105655) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The CRISPR knockout cell line was generated by EdiGene Inc.

Anti-GSTO1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105655

GTX105655 IHC-P Image

Immunohistochemical analysis of paraffin-embedded GSTA2 xenograft, using GSTO1(GTX105655) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GSTO1 antibody [N1C3] used in Western Blot (WB). GTX105655

GTX105655 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2 (GTX27900)
12% SDS PAGE
GTX105655 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSTO1 antibody [N1C3] used in Western Blot (WB). GTX105655

GTX105655 WB Image

Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with GSTO1 antibody [N1C3] (GTX105655) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169