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GSTO1 antibody [N1C3]

KO/KD
Anti-GSTO1 antibody [N1C3] used in Western Blot (WB). GTX105655
Anti-GSTO1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105655
Anti-GSTO1 antibody [N1C3] used in Western Blot (WB). GTX105655
Anti-GSTO1 antibody [N1C3] used in Western Blot (WB). GTX105655

Cat. No. GTX105655

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GSTO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

HepG2 , mouse colon , 293T

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GSTO1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037103

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutathione S-transferase omega 1 , GSTO 1-1 , GSTTLp28 , HEL-S-21 , P28 , SPG-R

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the theta class glutathione S-transferase-like (GSTTL) protein family. In mouse, the encoded protein acts as a small stress response protein, likely involved in cellular redox homeostasis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GSTO1 antibody [N1C3] used in Western Blot (WB). GTX105655

GTX105655 WB Image

KO/KD

Wild-type (WT) and GSTO1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GSTO1 antibody [N1C3] (GTX105655) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GSTO1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105655

GTX105655 IHC-P Image

Immunohistochemical analysis of paraffin-embedded GSTA2 xenograft, using GSTO1(GTX105655) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GSTO1 antibody [N1C3] used in Western Blot (WB). GTX105655

GTX105655 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with GSTO1 antibody [N1C3] (GTX105655) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSTO1 antibody [N1C3] used in Western Blot (WB). GTX105655

GTX105655 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
12% SDS PAGE
GTX105655 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Roberto Stella et al. Int J Mol Sci 2021; 22 (13) Perturbations of the Proteome and of Secreted Metabolites in Primary Astrocytes from the hSOD1(G93A) ALS Mouse Model.
Application : WB
Reactivity : Mouse
Xu Y et al. Cancer Res 2020; 80 (17) : 3692-3705 Deletion of Glutathione S-Transferase Omega 1 Activates Type I Interferon Genes and Downregulates Tissue Factor.
Application : WB
Reactivity : Human
Li Y et al. Tumour Biol 2014; Identification of glutathione S-transferase omega 1 (GSTO1) protein as a novel tumor-associated antigen and its autoantibody in human esophageal squamous cell carcinoma.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169