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GSTO1 antibody

KO/KD
Anti-GSTO1 antibody used in Western Blot (WB). GTX118439
Anti-GSTO1 antibody used in Western Blot (WB). GTX118439
Anti-GSTO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX118439
Anti-GSTO1 antibody used in Western Blot (WB). GTX118439
Anti-GSTO1 antibody used in Western Blot (WB). GTX118439

Cat. No. GTX118439

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GSTO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

HepG2 , mouse colon , rat liver , 293T

Predict Reactivity

Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GSTO1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174551

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutathione S-transferase omega 1 , GSTO 1-1 , GSTTLp28 , HEL-S-21 , P28 , SPG-R

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the theta class glutathione S-transferase-like (GSTTL) protein family. In mouse, the encoded protein acts as a small stress response protein, likely involved in cellular redox homeostasis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GSTO1 antibody used in Western Blot (WB). GTX118439

GTX118439 WB Image

KO/KD

Wild-type (WT) and GSTO1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GSTO1 antibody (GTX118439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSTO1 antibody used in Western Blot (WB). GTX118439

GTX118439 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
12% SDS PAGE
GTX118439 diluted at 1:5000

Anti-GSTO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX118439

GTX118439 IHC-P Image

GSTO1 antibody detects GSTO1 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
GSTO1 antibody (GTX118439) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GSTO1 antibody used in Western Blot (WB). GTX118439

GTX118439 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with GSTO1 antibody (GTX118439) diluted at 1:5000.

Anti-GSTO1 antibody used in Western Blot (WB). GTX118439

GTX118439 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with GSTO1 antibody (GTX118439) diluted at 1:5000.

REFERENCE

Application Reference

Alexander Hooftman et al. Cell Metab 2020; 32 (3) : 468-478 The Immunomodulatory Metabolite Itaconate Modifies NLRP3 and Inhibits Inflammasome Activation.
Application : WB
Reactivity : Mouse
Gueugneau M et al. BMC Genomics 2014; 15 (1) : 1165 Proteomics of muscle chronological ageing in post-menopausal women.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169