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GSTP1 antibody [N1N2], N-term

Anti-GSTP1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100299
Anti-GSTP1 antibody [N1N2], N-term used in Western Blot (WB). GTX100299
Anti-GSTP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100299
Anti-GSTP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100299

Cat No. GTX100299

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Dog, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GSTP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glutathione S-transferase pi 1,DFN7,FAEES3,GST3,GSTP,HEL-S-22,PI

Background

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-GSTP1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100299

GTX100299 ICC/IF Image

GSTP1 antibody [N1N2], N-term detects GSTP1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSTP1 protein stained by GSTP1 antibody [N1N2], N-term (GTX100299) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-GSTP1 antibody [N1N2], N-term used in Western Blot (WB). GTX100299

GTX100299 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX100299 diluted at 1:5000

Anti-GSTP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100299

GTX100299 IHC-P Image

GSTP1 antibody [N1N2], N-term detects GSTP1 protein at cytosol on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
GSTP1 antibody [N1N2], N-term (GTX100299) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GSTP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100299

GTX100299 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using GSTP1(GTX100299) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169