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GSTP1 antibody

Orthogonal
Anti-GSTP1 antibody used in Western Blot (WB). GTX112695
Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695
Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695
Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695
Anti-GSTP1 antibody used in Western Blot (WB). GTX112695
Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695
Anti-GSTP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112695
Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695
Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695
Anti-GSTP1 antibody used in Western Blot (WB). GTX112695
Anti-GSTP1 antibody used in Western Blot (WB). GTX112695
Anti-GSTP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112695

Cat. No. GTX112695

Reference 10

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GSTP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

Mouse brain , Rat brain , A549 , HCT116

Predict Reactivity

Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GSTP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10727212

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutathione S-transferase pi 1 , DFN7 , FAEES3 , GST3 , GSTP , HEL-S-22 , PI

Background

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GSTP1 antibody used in Western Blot (WB). GTX112695

GTX112695 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX112695 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695

GTX112695 IHC-P Image

GSTP1 antibody detects GSTP1 protein at nucleus on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
GSTP1 antibody (GTX112695) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695

GTX112695 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using GSTP1(GTX112695) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695

GTX112695 IHC-P Image

GSTP1 antibody detects GSTP1 protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
GSTP1 antibody (GTX112695) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GSTP1 antibody used in Western Blot (WB). GTX112695

GTX112695 WB Image

GSTP1 antibody detects GSTP1 protein by western blot analysis.
A. 50 μg Rat brain lysate/extract
12% SDS-PAGE
GSTP1 antibody (GTX112695) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695

GTX112695 IHC-P Image

GSTP1 antibody detects GSTP1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
GSTP1 antibody (GTX112695) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GSTP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112695

GTX112695 ICC/IF Image

GSTP1 antibody detects GSTP1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSTP1 protein stained by GSTP1 antibody (GTX112695) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695

GTX112695 IHC-P Image

GSTP1 antibody detects GSTP1 protein at nucleus on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
GSTP1 antibody (GTX112695) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695

GTX112695 IHC-P Image

GSTP1 antibody detects GSTP1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GSTP1 stained by GSTP1 antibody (GTX112695) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GSTP1 antibody used in Western Blot (WB). GTX112695

GTX112695 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GSTP1 antibody (GTX112695) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSTP1 antibody used in Western Blot (WB). GTX112695

GTX112695 WB Image

Orthogonal

The data was published in the journal J Exp Clin Cancer Res in 2019.PMID: 31324203

Anti-GSTP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112695

GTX112695 ICC/IF Image

The data was published in the journal J Exp Clin Cancer Res in 2019.PMID: 31324203

REFERENCE

Application Reference

E Bejarano et al. Redox Biol 2023; 66 : 102869 Unbalanced redox status network as an early pathological event in congenital cataracts
Application : WB, IHC-Fr
Reactivity : Mouse
Sze SCW et al. Int J Mol Sci 2022; 23 (20) Aberrant Transferrin and Ferritin Upregulation Elicits Iron Accumulation and Oxidative Inflammaging Causing Ferroptosis and Undermines Estradiol Biosynthesis in Aging Rat Ovaries by Upregulating NF-Κb-Activated Inducible Nitric Oxide Synthase: First Demonstration of an Intricate Mechanism.
Makoto Asahina et al. Mol Brain 2021; 14 (1) : 91 Reversibility of motor dysfunction in the rat model of NGLY1 deficiency.
Application : IHC-P
Reactivity : Rat
Asahina M et al. Hum Mol Genet 2020; 29 (10) : 1635-1647 Ngly1 -/- rats develop neurodegenerative phenotypes and pathological abnormalities in their peripheral and central nervous systems.
Zhang Q et al. J Exp Clin Cancer Res 2019; 38 (1) : 320 Exosomal transfer of p-STAT3 promotes acquired 5-FU resistance in colorectal cancer cells.
Application : WB, ICC/IF
Reactivity : Human
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Application Reference
E Bejarano et al. Redox Biol 2023; 66:102869 Unbalanced redox status network as an early pathological event in congenital cataracts
Application : WB, IHC-Fr
Reactivity : Mouse
Sze SCW et al. Int J Mol Sci 2022; 23 (20) Aberrant Transferrin and Ferritin Upregulation Elicits Iron Accumulation and Oxidative Inflammaging Causing Ferroptosis and Undermines Estradiol Biosynthesis in Aging Rat Ovaries by Upregulating NF-Κb-Activated Inducible Nitric Oxide Synthase: First Demonstration of an Intricate Mechanism.
Makoto Asahina et al. Mol Brain 2021; 14 (1):91 Reversibility of motor dysfunction in the rat model of NGLY1 deficiency.
Application : IHC-P
Reactivity : Rat
Asahina M et al. Hum Mol Genet 2020; 29 (10):1635-1647 Ngly1 -/- rats develop neurodegenerative phenotypes and pathological abnormalities in their peripheral and central nervous systems.
Zhang Q et al. J Exp Clin Cancer Res 2019; 38 (1):320 Exosomal transfer of p-STAT3 promotes acquired 5-FU resistance in colorectal cancer cells.
Application : WB, ICC/IF
Reactivity : Human
Kim HJ et al. Allergy Asthma Immunol Res 2019; 11 (4):560-571 Protease-Activated Receptors 2-Antagonist Suppresses Asthma by Inhibiting Reactive Oxygen Species-Thymic Stromal Lymphopoietin Inflammation and Epithelial Tight Junction Degradation.
Application : IHC-P
Reactivity : Mouse
Szu-Ting Lin et al. Cellular and Molecular Life Sciences 2015; 72 (12):2395-409 pgrmc1 contributes to *-induced chemoresistance in mes-sa uterine sarcoma.
Application : WB
Reactivity : Human
May EW et al. Pharmacol Res 2014; (Epub) identification of up- and down-regulated proteins in *-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of *-associated resistance.
Application : WB
Reactivity : Human
Chang JF et al. Toxicol Sci 2014; (Epub) nuclear proteomics with xrcc3 knockdown to reveal the development of *-resistant uterine cancer.
Application : WB
Reactivity : Human
Chen JY et al. J Nutr Biochem 2013; 24 (11):1889-910 High glucose-induced proteome alterations in hepatocytes and its possible relevance to diabetic liver disease.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169