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GSTP1 antibody

Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695
Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695
Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695
Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695
Anti-GSTP1 antibody used in Western Blot (WB). GTX112695
Anti-GSTP1 antibody used in Western Blot (WB). GTX112695
Anti-GSTP1 antibody used in Western Blot (WB). GTX112695
Anti-GSTP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112695
Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695

Cat No. GTX112695

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

A549 , HCT116 , Mouse brain , Rat brain

Predict Reactivity

Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GSTP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10727212

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glutathione S-transferase pi 1 , DFN7 , FAEES3 , GST3 , GSTP , HEL-S-22 , PI

Background

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695

GTX112695 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using GSTP1(GTX112695) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695

GTX112695 IHC-P Image

GSTP1 antibody detects GSTP1 protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
GSTP1 antibody (GTX112695) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695

GTX112695 IHC-P Image

GSTP1 antibody detects GSTP1 protein at nucleus on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
GSTP1 antibody (GTX112695) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695

GTX112695 IHC-P Image

GSTP1 antibody detects GSTP1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
GSTP1 antibody (GTX112695) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GSTP1 antibody used in Western Blot (WB). GTX112695

GTX112695 WB Image

GSTP1 antibody detects GSTP1 protein by western blot analysis.
A. 50 μg Rat brain lysate/extract
12% SDS-PAGE
GSTP1 antibody (GTX112695) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSTP1 antibody used in Western Blot (WB). GTX112695

GTX112695 WB Image

GSTP1 antibody detects GSTP1 protein by Western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg HCT116 whole cell lysate/extract
12 % SDS-PAGE
GSTP1 antibody (GTX112695) dilution: 1:5000

Anti-GSTP1 antibody used in Western Blot (WB). GTX112695

GTX112695 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX112695 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSTP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112695

GTX112695 ICC/IF Image

GSTP1 antibody detects GSTP1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSTP1 protein stained by GSTP1 antibody (GTX112695) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112695

GTX112695 IHC-P Image

GSTP1 antibody detects GSTP1 protein at nucleus on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
GSTP1 antibody (GTX112695) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

GSTP1 antibody Cat No. GTX112695

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2019.PMID: 31324203
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2019.PMID: 31324203
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf