GeneTex
United States (US)

GSTZ1 antibody [N1C3]

GTX106109_40150_IFA_19011506_257.jpg
GTX106109_IHC_19011506_713.jpg
GTX106109_WB_19011506_889.jpg

Cat No. GTX106109

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GSTZ1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glutathione S-Transferase Zeta 1,Gstz1-1,Maai,Maaid,Mai,Gstz1

Cellular Localization

Cytoplasm

Background

This gene is a member of the glutathione S-transferase (GSTs) super-family which encodes multifunctional enzymes important in the detoxification of electrophilic molecules, including carcinogens, mutagens, and several therapeutic drugs, by conjugation with glutathione. This enzyme also plays a significant role in the catabolism of phenylalanine and tyrosine. Thus defects in this enzyme may lead to severe metabolic disorders including alkaptonuria, phenylketonuria and tyrosinaemia. Several transcript variants of this gene encode multiple protein isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106109_40150_IFA_19011506_257.jpg

GTX106109 ICC/IF Image

GSTZ1 antibody [N1C3] detects GSTZ1 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: GSTZ1 protein stained by GSTZ1 antibody [N1C3] (GTX106109) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX106109_IHC_19011506_713.jpg

GTX106109 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MAAI(GTX106109) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106109_WB_19011506_889.jpg

GTX106109 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GTX106109 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169