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GSX2 antibody [HL2135]

Recombinant antibody
Anti-GSX2 antibody [HL2135] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638107
Anti-GSX2 antibody [HL2135] used in Western Blot (WB). GTX638107
Anti-GSX2 antibody [HL2135] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638107

Cat. No. GTX638107

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2135

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all GSX2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Product Note

This antibody is specific for human GSX2 protein, and it does not cross react with human GSX1 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human GSX2.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GS homeobox 2 , GSH2

Cellular Localization

Nucleus

Background

Enables DNA-binding transcription factor activity, RNA polymerase II-specific and sequence-specific double-stranded DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to act upstream of or within several processes, including nervous system development; positive regulation of Notch signaling pathway; and regulation of respiratory gaseous exchange by nervous system process. Located in cytoplasm and nucleus. [provided by Alliance of Genome Resources, Apr 2022]

Database

DATA IMAGES

Anti-GSX2 antibody [HL2135] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638107

GTX638107 ICC/IF Image

GSX2 antibody [HL2135] detects GSX2 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSX2 stained by GSX2 antibody [HL2135] (GTX638107) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GSX2 antibody [HL2135] used in Western Blot (WB). GTX638107

GTX638107 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with GSX2 antibody [HL2135] (GTX638107) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSX2 antibody [HL2135] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638107

GTX638107 ICC/IF Image

GSX2 antibody [HL2135] detects GSX2 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSX2 stained by GSX2 antibody [HL2135] (GTX638107) diluted at 1:250.
Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations