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GSX2 antibody [HL2533]

Recombinant antibody Overexpression
Anti-GSX2 antibody [HL2533] used in Western Blot (WB). GTX638902
Anti-GSX2 antibody [HL2533] used in IHC (Paraffin sections) (IHC-P). GTX638902
Anti-GSX2 antibody [HL2533] used in Western Blot (WB). GTX638902
Anti-GSX2 antibody [HL2533] used in Western Blot (WB). GTX638902

Cat. No. GTX638902

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2533

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all GSX2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Product Note

This antibody is specific for human GSX2 protein, and it does not cross react with human GSX1 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human GSX2

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GS homeobox 2 , GSH2

Cellular Localization

Nucleus

Background

Enables DNA-binding transcription factor activity, RNA polymerase II-specific and sequence-specific double-stranded DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to act upstream of or within several processes, including nervous system development; positive regulation of Notch signaling pathway; and regulation of respiratory gaseous exchange by nervous system process. Located in cytoplasm and nucleus. [provided by Alliance of Genome Resources, Apr 2022]

Database

DATA IMAGES

Anti-GSX2 antibody [HL2533] used in Western Blot (WB). GTX638902

GTX638902 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with GSX2 antibody [HL2533] (GTX638902) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GSX2 antibody [HL2533] used in IHC (Paraffin sections) (IHC-P). GTX638902

GTX638902 IHC-P Image

GSX2 antibody [HL2533] detects GSX2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 embryo.
GSX2 stained by GSX2 antibody [HL2533] (GTX638902) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GSX2 antibody [HL2533] used in Western Blot (WB). GTX638902

GTX638902 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with GSX2 antibody [HL2533] (GTX638902) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSX2 antibody [HL2533] used in Western Blot (WB). GTX638902

GTX638902 WB Image

Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with GSX2 antibody [HL2533] (GTX638902) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations