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GTF2A1 antibody

Anti-GTF2A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117420
Anti-GTF2A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX117420
Anti-GTF2A1 antibody used in Western Blot (WB). GTX117420

Cat. No. GTX117420

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GTF2A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , HeLa

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GTF2A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164039

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

general transcription factor IIA subunit 1 , TF2A1 , TFIIA , TFIIA-42 , TFIIAL

Cellular Localization

Nucleus

Background

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-GTF2A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117420

GTX117420 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GTF2A1(GTX117420) antibody at 1:500 dilution.

Anti-GTF2A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX117420

GTX117420 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using GTF2A1(GTX117420) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GTF2A1 antibody used in Western Blot (WB). GTX117420

GTX117420 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: HeLa
10% SDS PAGE
GTX117420 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169