GeneTex
United States (US)

GTF2H1 antibody [N1C1]

Comparison
GTX111804_40639_WB_18121410_832.jpg
GTX111804_40639_20180314_ICC_IF_18121410_707.jpg
GTX111804_40639_20161229_WB_competitor_watermark_18121410_413.jpg

Cat No. GTX111804

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116 , H1299

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GTF2H1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BTF2 antibody, TFB1 antibody, TFIIH antibody, GTF2H1 antibody, BTF2 p62 antibody, general transcription factor IIH subunit 1 antibody, general transcription factor IIH polypeptide 1 antibody, TFIIH basal transcription factor complex p62 subunit antibody, basic transcription factor 2 62 kDa subunit antibody, "general transcription factor IIH, polypeptide 1, 62kDa antibody"

Cellular Localization

Nucleus

Database

DATA IMAGES
GTX111804_40639_WB_18121410_832.jpg

GTX111804 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX111804 diluted at 1:1000

GTX111804_40639_20180314_ICC_IF_18121410_707.jpg

GTX111804 ICC/IF Image

GTF2H1 antibody [N1C1] detects GTF2H1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GTF2H1 stained by GTF2H1 antibody [N1C1] (GTX111804) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10μm.

GTX111804_40639_20161229_WB_competitor_watermark_18121410_413.jpg

GTX111804 WB Image

Comparison

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with GTF2H1 antibody [N1C1] (GTX111804) diluted at 1:1000 and competitor's antibody (SC-292) diluted by 1:200.
*The competitor is not affiliated with GeneTex and does not endorse this product.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169