APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , C2Cl2 , PC-12 , Rat2
Predict Reactivity
Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human GTPBP4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885291
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
GTP binding protein 4 , CRFG , NGB , NOG1
Cellular Localization
Nucleus , nucleolus
Background
GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX110825 ICC/IF Image
GTPBP4 antibody detects GTPBP4 protein at nucleolus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GTPBP4 protein stained by GTPBP4 antibody (GTX110825) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX110825 WB Image
GTPBP4 antibody detects GTPBP4 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
7.5 % SDS-PAGE
GTPBP4 antibody (GTX110825) dilution: 1:500
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GTX110825 IHC-P Image
GTPBP4 antibody detects GTPBP4 protein at nucleus and nucleolus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
GTPBP4 antibody (GTX110825) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX110825 WB Image
GTPBP4 antibody detects GTPBP4 protein by western blot analysis.
A 293T whole cell lysate/extract
B A431
C HeLa whole cell lysate/extract
D HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
GTPBP4 antibody (GTX110825) dilution: 1:1000
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GTX110825 WB Image
GTPBP4 antibody detects GTPBP4 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
GTPBP4 antibody (GTX110825) dilution: 1:500
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REFERENCE
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