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GTPBP4 antibody

Anti-GTPBP4 antibody used in Western Blot (WB). GTX110826
Anti-GTPBP4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110826
Anti-GTPBP4 antibody used in Western Blot (WB). GTX110826

Cat. No. GTX110826

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GTPBP4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GTPBP4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162395

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GTP binding protein 4 , CRFG , NGB , NOG1

Cellular Localization

Nucleus , nucleolus

Background

GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GTPBP4 antibody used in Western Blot (WB). GTX110826

GTX110826 WB Image

GTPBP4 antibody detects GTPBP4 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
GTPBP4 antibody (GTX110826) dilution: 1:5000

Anti-GTPBP4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110826

GTX110826 ICC/IF Image

GTPBP4 antibody detects GTPBP4 protein at nucleolus by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: GTPBP4 protein stained by GTPBP4 antibody (GTX110826) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-GTPBP4 antibody used in Western Blot (WB). GTX110826

GTX110826 WB Image

Sample (10 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX110826 diluted at 1:5000

REFERENCE

Application Reference

Issa A et al. Life Sci Alliance 2024; 7 (8) The nucleolar phase of signal recognition particle assembly.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169