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GUCY1A3 antibody [3G6B2]

Anti-GUCY1A3 antibody [3G6B2] used in Western Blot (WB). GTX60721
Anti-GUCY1A3 antibody [3G6B2] used in Western Blot (WB). GTX60721
Anti-GUCY1A3 antibody [3G6B2] used in Flow cytometry (FACS). GTX60721
Anti-GUCY1A3 antibody [3G6B2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60721
Anti-GUCY1A3 antibody [3G6B2] used in IHC (Paraffin sections) (IHC-P). GTX60721
Anti-GUCY1A3 antibody [3G6B2] used in IHC (Paraffin sections) (IHC-P). GTX60721
Anti-GUCY1A3 antibody [3G6B2] used in ELISA (ELISA). GTX60721

Cat. No. GTX60721

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3G6B2

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all GUCY1A3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human GUCY1A3 (AA: 22-214) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

guanylate cyclase 1 soluble subunit alpha 1 , GC-SA3 , GUC1A3 , GUCA3 , GUCSA3 , GUCY1A3 , MYMY6

Cellular Localization

Cytoplasm

Background

Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-GUCY1A3 antibody [3G6B2] used in Western Blot (WB). GTX60721

GTX60721 WB Image

WB analysis of HEK293 (1) and GUCY1A3 (AA: 22-214)-hIgGFc transfected HEK293 (2) cell lysate using GTX60721 GUCY1A3 antibody [3G6B2].

Anti-GUCY1A3 antibody [3G6B2] used in Western Blot (WB). GTX60721

GTX60721 WB Image

WB analysis of HEK293 (1) and Raji (2) cell lysate using GTX60721 GUCY1A3 antibody [3G6B2].

Anti-GUCY1A3 antibody [3G6B2] used in Flow cytometry (FACS). GTX60721

GTX60721 FACS Image

FACS analysis of HEK293 cells using GTX60721 GUCY1A3 antibody [3G6B2].
Green : GUCY1A3
Purple : negative control

Anti-GUCY1A3 antibody [3G6B2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60721

GTX60721 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60721 GUCY1A3 antibody [3G6B2].
Green : GUCY1A3
Blue: DRAQ5 fluorescent DNA dye

Anti-GUCY1A3 antibody [3G6B2] used in IHC (Paraffin sections) (IHC-P). GTX60721

GTX60721 IHC-P Image

IHC-P analysis of esophageal cancer tissue using GTX60721 GUCY1A3 antibody [3G6B2].

Anti-GUCY1A3 antibody [3G6B2] used in IHC (Paraffin sections) (IHC-P). GTX60721

GTX60721 IHC-P Image

IHC-P analysis of renal cancer tissue using GTX60721 GUCY1A3 antibody [3G6B2].

Anti-GUCY1A3 antibody [3G6B2] used in ELISA (ELISA). GTX60721

GTX60721 ELISA Image

ELISA analysis of antigen using GTX60721 GUCY1A3 antibody [3G6B2].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409