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GULP1 antibody [C2C3], C-term

Anti-GULP1 antibody [C2C3], C-term used in Western Blot (WB). GTX107740
Anti-GULP1 antibody [C2C3], C-term used in Western Blot (WB). GTX107740
Anti-GULP1 antibody [C2C3], C-term used in Western Blot (WB). GTX107740

Cat. No. GTX107740

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GULP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

HepG2 , Molt-4 , NIH-3T3 , 293T

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GULP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240924

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GULP PTB domain containing engulfment adaptor 1 , CED-6 , CED6 , GULP

Cellular Localization

Cytoplasm

Background

The prompt clearance of cells undergoing apoptosis is critical during embryonic development, normal tissue turnover, inflammation, and autoimmunity. CED6 is an evolutionarily conserved adaptor protein required for efficient engulfment of apoptotic cells by phagocytes.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-GULP1 antibody [C2C3], C-term used in Western Blot (WB). GTX107740

GTX107740 WB Image

Sample (30 ug of whole cell lysate)

A: Hep G2 (GTX27900)

B: Molt-4 (GTX27912)

10% SDS PAGE

GTX107740 diluted at 1:1000
Anti-GULP1 antibody [C2C3], C-term used in Western Blot (WB). GTX107740

GTX107740 WB Image

Sample (30 ug of whole cell lysate)

A: NIH-3T3

10% SDS PAGE

GULP1 antibody

GTX107740 diluted at 1:1000

Anti-GULP1 antibody [C2C3], C-term used in Western Blot (WB). GTX107740

GTX107740 WB Image

GULP1 antibody detects GULP1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with GULP1 antibody (GTX107740) diluted by 1:1000.

REFERENCE

Application Reference

Jha A et al. Mol Biol Cell 2012; 23 (9) : 1742-64 The apoptotic engulfment protein Ced-6 participates in clathrin-mediated yolk uptake in Drosophila egg chambers.
Application : WB
Reactivity : Rat
Beyer AS et al. Neurobiol Aging 2012; 33 (4) : 732-43 Engulfment adapter PTB domain containing 1 interacts with and affects processing of the amyloid-β precursor protein.
Application : IHC
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169