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Galactosidase alpha antibody [001]

Anti-Galactosidase alpha antibody [001] used in Western Blot (WB). GTX01987
Anti-Galactosidase alpha antibody [001] used in Immunoprecipitation (IP). GTX01987

Cat No. GTX01987

Host

Rabbit

Clonality

Monoclonal

Clone Name

001

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:5000
IP 4-6 μl/mg of lysate
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.2 μm filtered PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Human alpha-Galactosidase A / GLA protein

Purification

Purified by Protein A

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

galactosidase alpha , GALA

Cellular Localization

Lysosome

Background

This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Galactosidase alpha antibody [001] used in Western Blot (WB). GTX01987

GTX01987 WB Image

WB analysis of 293T whole cell lysate (Lane A) using GTX01987 Galactosidase alpha antibody [001].
Dilution : 1:1000
Loading : 30 μg

Anti-Galactosidase alpha antibody [001] used in Immunoprecipitation (IP). GTX01987

GTX01987 IP Image

IP analysis of MCF-7 whole cell lysate using GTX01987 Galactosidase alpha antibody [001].
IP antibody : 2 μl per 0.5 mg total whole cell lysate
Dilution : 1:1000
50 % Protein G agarose : 15 μl

REFERENCE

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REVIEW

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