Galactosidase alpha antibody [N1C2]

Name: Galactosidase alpha antibody [N1C2]
Brand: GeneTex
SKU: GTX101178
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Galactosidase alpha antibody [N1C2] used in Western Blot (WB). GTX101178

Anti-Galactosidase alpha antibody [N1C2] used in Immunoprecipitation (IP). GTX101178

Anti-Galactosidase alpha antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101178

Cat. No. GTX101178

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, IP
Reactivity	Human, Rat
Package	100 μl ($399), 25 μl ($169)

See all Galactosidase alpha products

View product citations for antibody GTX101178 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

293T , Rat lung

Product Note

Knockdown/Knockout validation was supported by customer review data.

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Galactosidase alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617397

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

galactosidase alpha , GALA

Cellular Localization

Lysosome

Background

This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties. [provided by RefSeq]

Database

Gene ID: 2717 GLA
UniProt: P06280 GLA

Research Area

DATA IMAGES

GTX101178 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Galactosidase alpha antibody [N1C2] (GTX101178) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101178 IP Image

Immunoprecipitation of GLA protein from 293T whole cell extracts using 5 μg of Galactosidase alpha antibody [N1C2] (GTX101178).
Western blot analysis was performed using Galactosidase alpha antibody [N1C2] (GTX101178) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101178 WB Image

Galactosidase alpha antibody [N1C2] detects Galactosidase alpha protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Galactosidase alpha antibody [N1C2] (GTX101178) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101178 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Galactosidase alpha(GTX101178) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Cui S et al. J Transl Med 2023; 21 (1) : 138 Modeling of Fabry disease nephropathy using patient derived human induced pluripotent stem cells and kidney organoid system.

Application : WB

Reactivity : Human

Ivanova MM et al. Biomolecules 2020; 10 (6) Rapid Clathrin-Mediated Uptake of Recombinant α-Gal-A to Lysosome Activates Autophagy.

Application : WB

Reactivity : Human

Nelson MP et al. Neurobiol Dis 2017; 110 : 68-81 The lysosomal enzyme alpha-Galactosidase A is deficient in Parkinson's disease brain in association with the pathologic accumulation of alpha-synuclein.

Application : WB

Reactivity : Human

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