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Galactosidase alpha antibody [N1C2]

Anti-Galactosidase alpha antibody [N1C2] used in Western Blot (WB). GTX101178
Anti-Galactosidase alpha antibody [N1C2] used in Immunoprecipitation (IP). GTX101178
Anti-Galactosidase alpha antibody [N1C2] used in Western Blot (WB). GTX101178
Anti-Galactosidase alpha antibody [N1C2] used in Western Blot (WB). GTX101178
Anti-Galactosidase alpha antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101178

Cat No. GTX101178

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

293T , HeLa , Rat Lung

Product Note

Knockdown/Knockout validation was supported by customer review data.

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.157 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Galactosidase alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617397

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

galactosidase alpha , GALA

Cellular Localization

Lysosome

Background

This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Galactosidase alpha antibody [N1C2] used in Western Blot (WB). GTX101178

GTX101178 WB Image

Galactosidase alpha antibody detects Galactosidase alpha protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Galactosidase alpha antibody (GTX101178) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galactosidase alpha antibody [N1C2] used in Immunoprecipitation (IP). GTX101178

GTX101178 IP Image

Immunoprecipitation of GLA protein from 293T whole cell extracts using 5 μg of Galactosidase alpha antibody [N1C2] (GTX101178).
Western blot analysis was performed using Galactosidase alpha antibody [N1C2] (GTX101178) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Galactosidase alpha antibody [N1C2] used in Western Blot (WB). GTX101178

GTX101178 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
10% SDS PAGE
Galactosidase alpha antibody
GTX101178 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galactosidase alpha antibody [N1C2] used in Western Blot (WB). GTX101178

GTX101178 WB Image

Galactosidase alpha antibody [N1C2] detects Galactosidase alpha protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Galactosidase alpha antibody [N1C2] (GTX101178) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galactosidase alpha antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101178

GTX101178 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Galactosidase alpha(GTX101178) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

Galactosidase alpha antibody [N1C2] Cat No. GTX101178

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 11-Oct-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 cells
Amount used : 50μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Lane Description : Lane 1: HEK293 cells, Lane 2: knockout of Galactosidase alpha in HEK293 cells
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169