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Galactosidase alpha antibody

Anti-Galactosidase alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32617
Anti-Galactosidase alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX32617
Anti-Galactosidase alpha antibody used in Western Blot (WB). GTX32617
Anti-Galactosidase alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX32617

Cat. No. GTX32617

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Galactosidase alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 150-429 of human GLA (NP_000160.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

galactosidase alpha , GALA

Cellular Localization

Lysosome

Background

This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Galactosidase alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32617

GTX32617 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32617 Galactosidase alpha antibody.
Blue : DAPI

Anti-Galactosidase alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX32617

GTX32617 IHC-P Image

IHC-P analysis of human liver tissue using GTX32617 Galactosidase alpha antibody.
Dilution : 1:100

Anti-Galactosidase alpha antibody used in Western Blot (WB). GTX32617

GTX32617 WB Image

WB analysis of various sample lysates using GTX32617 Galactosidase alpha antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Galactosidase alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX32617

GTX32617 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX32617 Galactosidase alpha antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399