GeneTex
  • Country / Location Selection

United States (US)

Galactosylceramidase antibody [N3C3]

Anti-Galactosylceramidase antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101197
Anti-Galactosylceramidase antibody [N3C3] used in Western Blot (WB). GTX101197

Cat No. GTX101197

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

Galactosylceramidase-transfected 293T

Predict Reactivity

Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.06 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Galactosylceramidase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

galactosylceramidase

Cellular Localization

Lysosome

Background

This gene encodes a lysosomal protein which hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Mutations in this gene have been associated with Krabbe disease, also known as globoid cell leukodystrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Galactosylceramidase antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101197

GTX101197 IHC-P Image

Galactosylceramidase antibody [N3C3] detects Galactosylceramidase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
Galactosylceramidase stained by Galactosylceramidase antibody [N3C3] (GTX101197) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Galactosylceramidase antibody [N3C3] used in Western Blot (WB). GTX101197

GTX101197 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Galactosylceramidase antibody [N3C3] (GTX101197) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
There are currently no references for Galactosylceramidase antibody [N3C3] (GTX101197). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Galactosylceramidase antibody [N3C3] (GTX101197). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169