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Galactosylceramidase antibody [N3C3]

Orthogonal Overexpression
Anti-Galactosylceramidase antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101197
Anti-Galactosylceramidase antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101197
Anti-Galactosylceramidase antibody [N3C3] used in Western Blot (WB). GTX101197
Anti-Galactosylceramidase antibody [N3C3] used in Western Blot (WB). GTX101197

Cat. No. GTX101197

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Galactosylceramidase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Predict Reactivity

Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Galactosylceramidase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037038

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

galactosylceramidase

Cellular Localization

Lysosome

Background

This gene encodes a lysosomal protein which hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Mutations in this gene have been associated with Krabbe disease, also known as globoid cell leukodystrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Galactosylceramidase antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101197

GTX101197 IHC-P Image

Galactosylceramidase antibody [N3C3] detects Galactosylceramidase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
Galactosylceramidase stained by Galactosylceramidase antibody [N3C3] (GTX101197) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Galactosylceramidase antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101197

GTX101197 IHC-P Image

Galactosylceramidase antibody [N3C3] detects Galactosylceramidase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Galactosylceramidase stained by Galactosylceramidase antibody [N3C3] (GTX101197) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Galactosylceramidase antibody [N3C3] used in Western Blot (WB). GTX101197

GTX101197 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Galactosylceramidase antibody [N3C3] (GTX101197) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galactosylceramidase antibody [N3C3] used in Western Blot (WB). GTX101197

GTX101197 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Galactosylceramidase antibody [N3C3] (GTX101197) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169