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Galectin 1 antibody [GAL1/1831]

Anti-Galectin 1 antibody [GAL1/1831] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34746
Anti-Galectin 1 antibody [GAL1/1831] used in IHC (Paraffin sections) (IHC-P). GTX34746
Anti-Galectin 1 antibody [GAL1/1831] used in Western Blot (WB). GTX34746
Anti-Galectin 1 antibody [GAL1/1831] used in Western Blot (WB). GTX34746
Anti-Galectin 1 antibody [GAL1/1831] used in Protein Array (Protein Array). GTX34746

Cat. No. GTX34746

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GAL1/1831

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, ELISA, Protein Array

Reactivity

 Human
Package
100 μg ($529)
See all Galectin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 min at RT
ELISA 2-4μg/ml (for coating)
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa12-108) of human Galectin-1 protein (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GAL1 , GBP , LGALS1 , galectin 1 , Galectin 1

Cellular Localization

Secreted,Extracellular space,Extracellular matrix

Background

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Galectin 1 antibody [GAL1/1831] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34746

GTX34746 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX34746 Galectin 1 antibody [GAL1/1831].
Green : Primary antibody
Red : nucleus

Anti-Galectin 1 antibody [GAL1/1831] used in IHC (Paraffin sections) (IHC-P). GTX34746

GTX34746 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX34746 Galectin 1 antibody [GAL1/1831].

Anti-Galectin 1 antibody [GAL1/1831] used in Western Blot (WB). GTX34746

GTX34746 WB Image

WB analysis of HeLa, K562, and 293 cell lysates using GTX34746 Galectin 1 antibody [GAL1/1831].

Anti-Galectin 1 antibody [GAL1/1831] used in Western Blot (WB). GTX34746

GTX34746 WB Image

WB analysis of HeLa cell lysate using GTX34746 Galectin 1 antibody [GAL1/1831].

Anti-Galectin 1 antibody [GAL1/1831] used in Protein Array (Protein Array). GTX34746

GTX34746 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using Galectin-1 Monospecific Mouse Monoclonal Antibody (GAL1/1831). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529