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Galectin 1 antibody [GAL1/1831]

Anti-Galectin 1 antibody [GAL1/1831] used in Western Blot (WB). GTX34746
Anti-Galectin 1 antibody [GAL1/1831] used in IHC (Paraffin sections) (IHC-P). GTX34746

Cat No. GTX34746

Host

Mouse

Clonality

Monoclonal

Clone Name

GAL1/1831

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT
FACS 0.5-1ug/million cells in 0.1ml

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Specificity/Sensitivity

Galectin-1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14kD participating in a variety of normal and pathological processes, including cancer progression. Galectin-1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin-1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 molecules present on the surface of lymphocytes. Galectin-1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant human Galectin-1 protein fragment (aa12-108) (exact sequence is proprietary)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Galectin 1 , Gal1 , Gbp , Lgals1

Background

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Galectin 1 antibody [GAL1/1831] used in Western Blot (WB). GTX34746

GTX34746 WB Image

Western Blot of HeLa, A431, K562 and 293 Cell Lysates using Galectin-1 Monoclonal Antibody (GAL1/1831).

Anti-Galectin 1 antibody [GAL1/1831] used in IHC (Paraffin sections) (IHC-P). GTX34746

GTX34746 IHC-P Image

Formalin-fixed, paraffin-embedded Human Prostate Carcinoma stained with Galectin-1 Monoclonal Antibody (GAL1/1831).

REFERENCE

There are currently no references for Galectin 1 antibody [GAL1/1831] (GTX34746). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Galectin 1 antibody [GAL1/1831] (GTX34746). Be the first to share your experience with this product.
Package List Price ($)
$ 429