GeneTex
United States (US)

Galectin 1 antibody

KO/KD
GTX101566_40744_20181026_WB_shRNA_watermark_19011519_568.jpg
GTX101566_40744_20161123_IFA_19011506_814.jpg
GTX101566_40744_WB_19011506_217.jpg
GTX101566_40744_20180316_WB_19011506_935.jpg
GTX101566_40744_IHC_19011506_666.jpg
GTX101566_40744_20161110_WB_R_19011506_357.jpg
GTX101566_40744_WB_M_19011506_146.jpg
GTX101566_40744_20161005_IHC-Fr_R_19011506_423.jpg

Cat No. GTX101566

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC-Wm Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

K562 , THP-1 , HeLa , NCI-H929 , BCL-1 , PC-12 , Rat2 , *U2OS

Predict Reactivity

Sheep, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Galectin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp686E23103 antibody, GAL1 antibody, GBP antibody, LGALS1 antibody, HPL antibody, gal-1 antibody, lactose-binding lectin 1 antibody, galaptin antibody, 14 kDa laminin-binding protein antibody, HLBP14 antibody, S-Lac lectin 1 antibody, beta-galactoside-binding lectin L-14-I antibody, galectin-1 antibody, 14 kDa lectin antibody, HBL antibody, putative MAPK-activating protein PM12 antibody, galectin 1 antibody, beta-galactoside-binding protein 14kDa antibody, "lectin, galactoside-binding, soluble, 1 antibody"

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101566_40744_20181026_WB_shRNA_watermark_19011519_568.jpg

GTX101566 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX101566) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101566_40744_20161123_IFA_19011506_814.jpg

GTX101566 ICC/IF Image

Galectin 1 antibody detects Galectin 1 protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Galectin 1 protein stained by Galectin 1 antibody (GTX101566) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX101566_40744_WB_19011506_217.jpg

GTX101566 WB Image

Sample (30 µg of whole cell lysate)
A: NCI-H929
15% SDS PAGE
GTX101566 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101566_40744_20180316_WB_19011506_935.jpg

GTX101566 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX101566) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101566_40744_IHC_19011506_666.jpg

GTX101566 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Galectin 1(GTX101566) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101566_40744_20161110_WB_R_19011506_357.jpg

GTX101566 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX101566) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101566_40744_WB_M_19011506_146.jpg

GTX101566 WB Image

Sample (30 µg of whole cell lysate)
A: BCL-1
15% SDS PAGE
GTX101566 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101566_40744_20161005_IHC-Fr_R_19011506_423.jpg

GTX101566 IHC-Fr Image

Galectin 1 antibody detects Galectin 1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Galectin 1 protein stained by Galectin 1 antibody (GTX101566) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE
REVIEW

Galectin 1 antibody Cat No. GTX101566

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 07-Jul-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 10μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169