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Galectin 1 antibody

Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566
Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566
Anti-Galectin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101566
Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566
Anti-Galectin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101566
Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566
Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566
Anti-Galectin 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX101566

Cat No. GTX101566

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC-Wm Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

K562 , THP-1 , HeLa , NCI-H929 , BCL-1 , PC-12 , Rat2 , *U2OS

Predict Reactivity

Sheep, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Galectin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

galectin 1 , GAL1 , GBP

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566

GTX101566 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX101566) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566

GTX101566 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX101566) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Galectin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101566

GTX101566 ICC/IF Image

Galectin 1 antibody detects Galectin 1 protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Galectin 1 protein stained by Galectin 1 antibody (GTX101566) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566

GTX101566 WB Image

Sample (30 µg of whole cell lysate)
A: NCI-H929
15% SDS PAGE
GTX101566 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101566

GTX101566 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Galectin 1(GTX101566) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566

GTX101566 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX101566) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566

GTX101566 WB Image

Sample (30 µg of whole cell lysate)
A: BCL-1
15% SDS PAGE
GTX101566 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX101566

GTX101566 IHC-Fr Image

Galectin 1 antibody detects Galectin 1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Galectin 1 protein stained by Galectin 1 antibody (GTX101566) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE
REVIEW

Galectin 1 antibody Cat No. GTX101566

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 07-Jul-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 10μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169