GeneTex

  • Country / Location Selection

United States (US)

Galectin 1 antibody

KO/KD Orthogonal
Anti-Galectin 1 antibody used in Western Blot (WB). GTX116411
Anti-Galectin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116411
Anti-Galectin 1 antibody used in Western Blot (WB). GTX116411
Anti-Galectin 1 antibody used in Immunoprecipitation (IP). GTX116411
Anti-Galectin 1 antibody used in Western Blot (WB). GTX116411
Anti-Galectin 1 antibody used in Western Blot (WB). GTX116411
Anti-Galectin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116411
Anti-Galectin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116411
Anti-Galectin 1 antibody used in Western Blot (WB). GTX116411

Cat. No. GTX116411

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Galectin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

NCI-H929 , BCL-1 , PC-12 , Rat2 , 293T , K562 , THP-1 , HL-60

Predict Reactivity

Sheep, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Galectin 1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624275

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

galectin 1 , GAL1 , GBP

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Galectin 1 antibody used in Western Blot (WB). GTX116411

GTX116411 WB Image

Sample (30 μg of whole cell lysate)
A: NCI-H929
15% SDS PAGE
GTX116411 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116411

GTX116411 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using Galectin 1(GTX116411) antibody at 1:200 dilution.

Anti-Galectin 1 antibody used in Western Blot (WB). GTX116411

GTX116411 WB Image

Galectin 1 antibody detects Galectin 1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
15% SDS-PAGE
Galectin 1 antibody (GTX116411) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 1 antibody used in Immunoprecipitation (IP). GTX116411

GTX116411 IP Image

Galectin 1 antibody immunoprecipitates Galectin 1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Galectin 1 antibody expressing HeLa cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Galectin 1 by 2.5 μg of Galectin 1 antibody (GTX116411) 15% SDS-PAGE The immunoprecipitated Galectin 1 protein was detected by Galectin 1 antibody (GTX116411) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Galectin 1 antibody used in Western Blot (WB). GTX116411

GTX116411 WB Image

Sample (30 μg of whole cell lysate)
A: BCL-1
15% SDS PAGE
GTX116411 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 1 antibody used in Western Blot (WB). GTX116411

GTX116411 WB Image

KO/KD

Wild-type (WT) and Galectin 1 knockout (KO) 293T cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX116411) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Galectin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116411

GTX116411 ICC/IF Image

Galectin 1 antibody detects Galectin 1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Galectin 1 protein stained by Galectin 1 antibody (GTX116411) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-Galectin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116411

GTX116411 ICC/IF Image

Galectin 1 antibody detects Galectin 1 protein at cytoplasm by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Galectin 1 protein stained by Galectin 1 antibody (GTX116411) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-Galectin 1 antibody used in Western Blot (WB). GTX116411

GTX116411 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX116411) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Paz H et al. J Exp Clin Cancer Res 2018; 37 (1) : 67 Treatment of B-cell precursor acute lymphoblastic leukemia with the Galectin-1 inhibitor PTX008.
Application : WB
Reactivity : Human
Mori Y et al. J Biol Chem 2015; 290 (43) : 26125-40 Binding of Galectin-3, a β-Galactoside-binding Lectin, to MUC1 Protein Enhances Phosphorylation of Extracellular Signal-regulated Kinase 1/2 (ERK1/2) and Akt, Promoting Tumor Cell Malignancy.
Application : ICC/IF
Reactivity : Human
May EW et al. Pharmacol Res 2014; (Epub) identification of up- and down-regulated proteins in *-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of *-associated resistance.
Application : WB
Reactivity : Human
Lin LH et al. Electrophoresis 2014; Biomarker discovery for neuroendocrine cervical cancer.
Application : WB
Reactivity : Human
Fan CY et al. Electrophoresis 2013; 34 (24) : 3305-14 Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Galectin 1 antibody (GTX116411). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169