Galectin 1 antibody detects Galectin 1 protein at cytoplasm by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Galectin 1 protein stained by Galectin 1 antibody (GTX116411) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

Galectin 1 antibody detects Galectin 1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Galectin 1 protein stained by Galectin 1 antibody (GTX116411) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

Wild-type (WT) and Galectin 1 knockout (KO) 293T cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX116411) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Sample (30 μg of whole cell lysate)
A: BCL-1
15% SDS PAGE
GTX116411 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Galectin 1 antibody immunoprecipitates Galectin 1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Galectin 1 antibody expressing HeLa cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Galectin 1 by 2.5 μg of Galectin 1 antibody (GTX116411) 15% SDS-PAGE The immunoprecipitated Galectin 1 protein was detected by Galectin 1 antibody (GTX116411) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Galectin 1 antibody detects Galectin 1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
15% SDS-PAGE
Galectin 1 antibody (GTX116411) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed A431, using Galectin 1(GTX116411) antibody at 1:200 dilution.

Sample (30 μg of whole cell lysate)
A: NCI-H929
15% SDS PAGE
GTX116411 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX116411) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.