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Galectin 3 antibody [01]

Anti-Galectin 3 antibody [01] used in Immunoprecipitation (IP). GTX02017
Anti-Galectin 3 antibody [01] used in Western Blot (WB). GTX02017

Cat No. GTX02017

Host

Mouse

Clonality

Monoclonal

Clone Name

01

Isotype

IgG1

Application

WB, IP

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
IP 1-4 μl/mg of lysate
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Product Note

No cross-reactivity with human Galectin1 protein and human Galectin8 protein in ELISA assay.

PROPERTIES

Form

Liquid

Buffer

0.2 μm filtered PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Human Galectin-3 / LGALS3 protein

Purification

Purified by Protein A

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

galectin 3 , CBP35 , GAL3 , GALBP , GALIG , L31 , LGALS2 , MAC2

Cellular Localization

Cytoplasm , Nucleus , Secreted

Background

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

Database

Research Area

DATA IMAGES

Anti-Galectin 3 antibody [01] used in Immunoprecipitation (IP). GTX02017

GTX02017 IP Image

IP analysis of MCF-7 whole cell lysate using GTX02017 Galectin 3 antibody [01].
IP antibody : 4 μl per 0.5 mg total whole cell lysate
Dilution : 1:100
50 % Protein G agarose : 15 μl

Anti-Galectin 3 antibody [01] used in Western Blot (WB). GTX02017

GTX02017 WB Image

WB analysis of various samples using GTX02017 Galectin 3 antibody [01].
Lane A : HeLa whole cell lysate
Lane B : SW480 whole cell lysate
Lane C : A431 whole cell lysate
Lane D : MCF-7 whole cell lysate
Dilution : 1:500
Loading : 30 μg

REFERENCE

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REVIEW

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